Hassan Mohamed I, Hassan Saber S, Soliman Farid N K, Khalil Mohamed H
Livestock Research Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab, Alexandria, Egypt.
Animal and Poultry Production Department, Faculty of Agriculture, Damanhour University, Damanhour, Egypt.
J Anim Physiol Anim Nutr (Berl). 2025 Mar;109(2):259-267. doi: 10.1111/jpn.14052. Epub 2024 Oct 5.
Royal jelly renowned for its robust nutritional, functional, and biological properties, is a pivotal product derived from honeybees. The purpose of this investigation was to assess the theory that in ovo injection of freeze-dried royal jelly (FDRJ) solutions at varying concentrations can influence the hatchability, blood properties and hatching characteristics of day-old chicks. A total of 480 eggs (54.81 ± 0.187 g) were allocated into four experimental groups: negative control (NC), without injection, positive control (PC), administered with regular saline, a low FDRJ dose group (9 mg/egg), and a high FDRJ dose group (18 mg/egg). The in ovo injections were administered on Day 18 of incubation, and the experiment was subsequently continued until the incubation period concluded at 21 days. Results revealed that the lower FDRJ dose (9 mg/egg) significantly improved hatchability percentages compared to other treatments. Conversely, the higher FDRJ dose (18 mg/egg) and control groups (NC and PC) resulted in significantly higher chick yield percentages than the lower FDRJ and PC groups. The NC group showed the supreme yolk sac (YS) percentage, whereas the yolk-free body mass (YFBM) percentages displayed an inverse trend. Furthermore, the in ovo FDRJ injection did not affect haematological values or the relative organ weight of day-old chicks. In conclusion, in ovo FDRJ injection demonstrated beneficial effects on hatchability and chick weight, as evidenced by the studied parameters.
蜂王浆以其丰富的营养、功能和生物学特性而闻名，是一种源自蜜蜂的关键产品。本研究的目的是评估以下理论：在孵化过程中向鸡蛋内注射不同浓度的冻干蜂王浆（FDRJ）溶液会影响一日龄雏鸡的孵化率、血液特性和孵化特征。总共480枚鸡蛋（54.81±0.187克）被分为四个实验组：阴性对照组（NC），不进行注射；阳性对照组（PC），注射生理盐水；低剂量FDRJ组（9毫克/枚鸡蛋）；高剂量FDRJ组（18毫克/枚鸡蛋）。在孵化的第18天进行鸡蛋内注射，随后实验持续进行至21天孵化期结束。结果显示，与其他处理组相比，较低剂量的FDRJ（9毫克/枚鸡蛋）显著提高了孵化率。相反，较高剂量的FDRJ（18毫克/枚鸡蛋）组和对照组（NC和PC）的雏鸡产出率显著高于低剂量FDRJ组和PC组。NC组的卵黄囊（YS）百分比最高，而无卵黄体重（YFBM）百分比呈现相反趋势。此外，鸡蛋内注射FDRJ对一日龄雏鸡的血液学值或相对器官重量没有影响。总之，研究参数表明，鸡蛋内注射FDRJ对孵化率和雏鸡体重有有益影响。
