过表达 TdNACB 可提高水稻的抗旱性。

Overexpression of TdNACB improves the drought resistance of rice.

机构信息

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, China; Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province, Horticulture Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.

出版信息

Plant Physiol Biochem. 2024 Nov;216:109157. doi: 10.1016/j.plaphy.2024.109157. Epub 2024 Sep 27.

DOI:10.1016/j.plaphy.2024.109157

PMID:39369649

Abstract

Drought stress greatly affects disrupts the productivity, ecological structure, physiological and biochemical activities of wheat at different growth stages. However, drought stress tolerance is a complex quantitative trait and involves multiple metabolic pathways. We found that a wild emmer introgression line BAd7-209 had stronger drought resistance compared with drought resistant wheat Zhongmai 175. The transcriptome analysis found 14,284, 22,383 and 21,451 genes had expression corresponding responsed to drought stress at 24h, 48h, 120h, respectively and significantly enriched in 'Arginine and proline metabolism' and 'Peroxisome' in BAd7-209. 1666 transcription factors (TFs) related responsed to drought stress in which TdNACB showed high expression at 24h, 48h and 120h and had the closest relationship with TaNAC48 and OsNAC6 in phylogenetic analysis. Overexpression of TdNACB significantly enhanced drought resistance in rice and overexpression lines had significantly higher CAT, POD and SOD activity, Pro content and lower MDA content than those of the WT under drought stress. The result demonstrated that TdNACB positively regulates drought resistance through increasing proline content and enhancing activity of enzyme related to ROS scavenging. The results of this study provides candidate genes for improving wheat drought resistance and guide as reference for studying the molecular mechanisms of wheat drought resistance.

摘要

干旱胁迫极大地影响了小麦在不同生长阶段的生产力、生态结构、生理生化活动。然而，抗旱性是一个复杂的数量性状，涉及多个代谢途径。我们发现，一个野生硬粒小麦导入系 BAd7-209 比抗旱小麦中麦 175 具有更强的抗旱性。转录组分析发现，在 24h、48h 和 120h 时，BAd7-209 中有 14284、22383 和 21451 个基因的表达对应于干旱胁迫，并且在 '精氨酸和脯氨酸代谢' 和 '过氧化物酶体' 中显著富集。有 1666 个转录因子（TFs）与干旱胁迫相关，其中 TdNACB 在 24h、48h 和 120h 时表达量较高，在系统发育分析中与 TaNAC48 和 OsNAC6 关系最密切。TdNACB 的过表达显著提高了水稻的抗旱性，在干旱胁迫下，过表达系的 CAT、POD 和 SOD 活性、脯氨酸含量显著高于 WT，MDA 含量显著低于 WT。结果表明，TdNACB 通过增加脯氨酸含量和增强与 ROS 清除相关的酶活性来正向调节抗旱性。本研究结果为提高小麦抗旱性提供了候选基因，并为研究小麦抗旱性的分子机制提供了参考。