Université de Reims-Champagne-Ardenne, INRAE, FARE, UMR A 614, Reims 51100, France.
Platform of Cellular and Tissular Imaging (PICT), Université de Reims Champagne Ardenne, 51100 Reims, France.
Bioresour Technol. 2024 Dec;414:131551. doi: 10.1016/j.biortech.2024.131551. Epub 2024 Oct 5.
Understanding lignocellulosic biomass resistance to enzymatic deconstruction is crucial for its sustainable conversion into bioproducts. Despite scientific advances, quantitative morphological analysis of plant deconstruction at cell and tissue scales remains under-explored. In this study, an original pipeline is devised, involving four-dimensional (space + time) fluorescence confocal imaging, and a novel computational tool, to track and quantify deconstruction at cell and tissue scales. By applying this pipeline to poplar wood, dynamics of cellular parameters was computed and cellulose conversion during enzymatic deconstruction was measured. Results showed that enzymatic deconstruction predominantly impacts cell wall volume rather than surface area. Additionally, a negative correlation was observed between pre-hydrolysis compactness measures and volumetric cell wall deconstruction rate, whose strength was modulated by enzymatic activity. Results also revealed a strong positive correlation between average volumetric cell wall deconstruction rate and cellulose conversion rate. These findings link key deconstruction parameters across nano and micro scales.
理解木质纤维素生物质对酶解的抗性对于其可持续转化为生物制品至关重要。尽管取得了科学进展，但在细胞和组织尺度上对植物解构的定量形态分析仍未得到充分探索。在这项研究中，设计了一个包含四维（空间+时间）荧光共焦成像和一种新的计算工具的原始管道，以在细胞和组织尺度上跟踪和量化解构。通过将该管道应用于杨树木材，计算了细胞参数的动力学，并测量了酶解过程中纤维素的转化。结果表明，酶解主要影响细胞壁体积而不是表面积。此外，在预水解紧密度测量值和体积细胞壁解构速率之间观察到负相关，其强度受酶活性调节。结果还表明，平均体积细胞壁解构速率与纤维素转化率之间存在很强的正相关关系。这些发现将纳米和微观尺度上的关键解构参数联系起来。
