Magalhães J B, Pontes A L, Andrade S G
Mem Inst Oswaldo Cruz. 1985 Jan-Mar;80(1):41-50. doi: 10.1590/s0074-02761985000100007.
The behavior of two strains of Trypanosoma cruzi (Y and Peruvian strains) in experimental mouse infection, after being passed through different conditions of maintainance and cultivation was studied. The conditions were: Warren's acellular culture medium, cryopreservation in liquid Nitrogen, passage through the insect vector and direct blood passage from mice to mice. The parameters considered for comparative study were as follows: parasitemia, mortality rate, maximum survival time, morphology of parasites in peripheral blood, tissue tropism and histopathological lesions. Each experimental group consisted of two sub-groups according to the inocula: 10.000 or 50.000 trypomastigotes. The basic characteristics of the strains remained unchanged. These were macrophagotropism, predominance of slender forms of the parasite in early infection and 100 per cent mortality rate in the acute phase of the infection. However, decrease in the virulence was observed when the culture forms were used or when the infection with low inoculum was used (10.00 forms). Therefore the main biological characteristics of the strains tended to remain the same, regardless of the conditions used for maintainance and cultivation.
研究了两株克氏锥虫（Y株和秘鲁株）在经过不同保存和培养条件后，在实验性小鼠感染中的行为。这些条件包括：沃伦无细胞培养基、液氮冷冻保存、通过昆虫媒介传代以及从小鼠到小鼠的直接血液传代。用于比较研究的参数如下：寄生虫血症、死亡率、最大存活时间、外周血中寄生虫的形态、组织嗜性和组织病理学损伤。根据接种量，每个实验组由两个亚组组成：10000或50000个锥鞭毛体。菌株的基本特征保持不变。这些特征包括巨噬细胞嗜性、感染早期寄生虫细长形态占优势以及感染急性期100%的死亡率。然而，当使用培养形式或低接种量感染（10000个形式）时，观察到毒力下降。因此，无论用于保存和培养的条件如何，菌株的主要生物学特征倾向于保持不变。
