Shu Gang, Tang Ziting, Du Hong, Zheng Yilei, Chang Lijen, Li Haohuan, Xu Funeng, Fu Hualin, Zhang Wei, Lin Juchun
Department of Basic Veterinary Medicine, Sichuan Agricultural University, Chengdu 611100, China.
Department of Veterinary Medicine, University of Minnesota, Minneapolis, MN 55791, USA.
Toxins (Basel). 2022 Mar 21;14(3):227. doi: 10.3390/toxins14030227.
Lipopolysaccharide (LPS) is an endotoxin that can cause an imbalance between the oxidation and antioxidant defense systems and then induces hepatic damages. Ferulic acid (FA) has multiple biological functions including antibacterial and antioxidant activities; however, the effect of FA on lipopolysaccharide-induced hepatic injury remains unknown. The purpose of this study was to investigate the mechanism of action of dietary Ferulic acid against Lipopolysaccharide-induced hepatic injuries in Tianfu broiler chickens. The results showed that supplementation of FA in daily feed increased body weight (BW) and decreased the feed conversion ratio (FCR) in LPS treatment broilers significantly (p < 0.05). Additionally, supplement of FA alleviated histological changes and apoptosis of hepatocytes in LPS treatment broilers. Supplement of FA significantly decreases the activities of ROS. Interestingly, the levels of antioxidant parameters including total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), and glutathione (GSH) in LPS group were significantly increased by the FA supplementation (p < 0.05). Nevertheless, administration of LPS to broilers decreased the expressions of Nrf2, NQO1, SOD, GSH-Px, CAT and Bcl-2, whereas it increased the expressions of Bax and Caspase-3 (p < 0.05). Moreover, the expressions of Nrf2, NQO1, SOD, CAT, Bcl-2 were significantly upregulated and Caspase-3 were significantly downregulated in the FL group when compared to LPS group (p < 0.05). In conclusion, supplementation of FA in daily feed improves growth performance and alleviates LPS-induced oxidative stress, histopathologic changes, and apoptosis of hepatocytes in Tianfu broilers.
脂多糖（LPS）是一种内毒素，可导致氧化与抗氧化防御系统失衡，进而引发肝脏损伤。阿魏酸（FA）具有多种生物学功能，包括抗菌和抗氧化活性；然而，FA对脂多糖诱导的肝损伤的影响尚不清楚。本研究旨在探讨日粮阿魏酸对天府肉鸡脂多糖诱导的肝损伤的作用机制。结果表明，在LPS处理的肉鸡日粮中添加FA可显著增加体重（BW）并降低饲料转化率（FCR）（p<0.05）。此外，添加FA可减轻LPS处理肉鸡的组织学变化和肝细胞凋亡。添加FA可显著降低活性氧（ROS）的活性。有趣的是，添加FA可显著提高LPS组中包括总超氧化物歧化酶（T-SOD）、总抗氧化能力（T-AOC）和谷胱甘肽（GSH）在内的抗氧化参数水平（p<0.05）。然而，给肉鸡注射LPS会降低Nrf2、NQO1、SOD、GSH-Px、CAT和Bcl-2的表达，而增加Bax和Caspase-3的表达（p<0.05）。此外，与LPS组相比，FL组中Nrf2、NQO1、SOD、CAT、Bcl-2的表达显著上调，Caspase-3的表达显著下调（p<0.05）。总之，在天府肉鸡日粮中添加FA可提高生长性能，减轻LPS诱导的氧化应激、组织病理学变化和肝细胞凋亡。
