Huang Jinyi, Ullah Ihsan, Chen Linjie, Peng Ke, Ou Yujie, Gong Zehua, Wu Aimin, He Zhimin, Yin Wenyan, Li Huaqiong
Joint Centre of Translational Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, P. R. China.
Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 325011, P. R. China.
Adv Healthc Mater. 2025 Mar;14(8):e2402393. doi: 10.1002/adhm.202402393. Epub 2024 Oct 6.
In wound healing and clinical anti-infection therapy, the current feasibility of nanocatalysts is extremely limited because of inadequate reactive oxygen species (ROS) generation. Herein, a novel HO self-supplying nanocomposite (M/C/AEK) consists of molybdenum disulfide (MoS) decorated with calcium peroxide (CaO) prepared at ambient temperature and encapsulated in AEK hydrogel. In the presence of HO and poly(vinyl pyrrolidone) (PVP), CaO nanoclusters, ≈30 nm, are anchored on the MoS surface. MoS/CaO can induce both a cascaded peroxidase (POD)-like and catalase (CAT)-like catalytic activity to produce toxic hydroxyl radicals through self-supplied HO and O responsive to the faintly acidic environment of acute wounds. The POD-like activity is increased under acidic compared with neutral conditions, allowing selective treatment of acute, slightly acidic wounds while avoiding the side effects of high-concentration antibacterial agents on normal tissues. The high near-infrared photothermal effect synergistically with POD-like/CAT-like activity of MoS/CaO boosts the production of more ROS to eradicate Staphylococcus aureus and Escherichia coli bacteria (98.6% and 98.9%) effectively and selectively stimulate wound healing. The porous M/C/AEK hydrogel in the wound microenvironment can efficiently capture bacteria, and its Ca ions and keratin stimulate healing, revealing excellent potential in advanced wound care and infection control therapies.
在伤口愈合和临床抗感染治疗中，由于活性氧（ROS）生成不足，目前纳米催化剂的可行性极其有限。在此，一种新型的自供应羟基纳米复合材料（M/C/AEK）由在室温下制备并封装在AEK水凝胶中的过氧化钙（CaO）修饰的二硫化钼（MoS₂）组成。在羟基（HO）和聚乙烯吡咯烷酮（PVP）存在的情况下，约30纳米的CaO纳米团簇锚定在MoS₂表面。MoS₂/CaO能够诱导级联的类过氧化物酶（POD）和类过氧化氢酶（CAT）催化活性，通过对急性伤口微酸性环境响应的自供应HO和O₂产生有毒的羟基自由基。与中性条件相比，酸性条件下类POD活性增强，从而能够选择性治疗急性、微酸性伤口，同时避免高浓度抗菌剂对正常组织的副作用。MoS₂/CaO的高近红外光热效应与类POD/类CAT活性协同作用，促进产生更多的ROS，有效且选择性地根除金黄色葡萄球菌和大肠杆菌（分别为98.6%和98.9%），并刺激伤口愈合。伤口微环境中的多孔M/C/AEK水凝胶能够有效捕获细菌，其钙离子和角蛋白可刺激愈合，在先进的伤口护理和感染控制治疗中显示出优异的潜力。
