Gani Riehana, Sheikh Bashir Ahmad, Mir Rakeeb Ahmad, Bhat Basharat Ahmad, Dar Pervaize Ahmad, Mohany Mohamed, Dar Mohd Younis, Bhat Zulfiqar Ali
Regional Research Institute of Unani and Ayurvedic Medicine (RRIUM), University of Kashmir, Srinagar, India.
Department of Bio-Resources, School of Biological Sciences, University of Kashmir, Srinagar, India.
Nat Prod Res. 2024 Oct 7:1-11. doi: 10.1080/14786419.2024.2412303.
There has been increasing interest in biologically active plant extracts. Studies continue to discover novel components, especially those with anti-anxiety activities. The present study investigates the anxiolytic activity of Wall. ex Benth. aerial parts through both and studies. Aerial parts of the experimental plant were extracted using a hydroalcoholic solvent and fractionated with various organic solvents of differing polarities, including hexane, dichloromethane, ethyl acetate, and n-butanol. The chemical compositions were determined using gas chromatography-mass spectrometry (GC/MS). anti-anxiety activity was tested on various Swiss albino mice models. Results indicate that all fractions of exhibited significant anxiolytic effects, with the butanol fraction displaying the highest efficacy. Molecular docking analysis suggested that some of the compounds could target anxiety disorder proteins. ADME/T calculations were performed to examine the effects of extracts on human metabolism. Therefore, the present study establishes the significant anti-anxiety activity of aerial parts, suggesting its potential as a therapeutic agent for various anxiety disorders.
人们对具有生物活性的植物提取物越来越感兴趣。研究不断发现新的成分，尤其是那些具有抗焦虑活性的成分。本研究通过行为学和生化研究来探究Wall. ex Benth.地上部分的抗焦虑活性。实验植物的地上部分用氢醇溶剂提取，并用不同极性的各种有机溶剂（包括己烷、二氯甲烷、乙酸乙酯和正丁醇）进行分离。使用气相色谱 - 质谱联用仪（GC/MS）测定化学成分。在各种瑞士白化小鼠模型上测试其抗焦虑活性。结果表明，Wall. ex Benth.的所有馏分均表现出显著的抗焦虑作用，其中丁醇馏分显示出最高的功效。分子对接分析表明，一些化合物可能靶向焦虑症相关蛋白。进行了ADME/T计算以检查Wall. ex Benth.提取物对人体代谢的影响。因此，本研究证实了Wall. ex Benth.地上部分具有显著的抗焦虑活性，表明其作为各种焦虑症治疗药物的潜力。
