Cell Technologies, Transplantation and Research Department, National Scientific Medical Center, Astana, Kazakhstan.
PerciaVista R&D Co. Shiraz, Iran.
Iran J Med Sci. 2024 Sep 1;49(9):559-572. doi: 10.30476/ijms.2023.99613.3172. eCollection 2024 Sep.
Primary biliary cholangitis (PBC) is a condition affecting the liver and immune system. In this study, the impact of autologous bone marrow-derived mononuclear cell (BM-MNC) transplantation on PBC patients was investigated.
Sixteen eligible PBC patients participated at the National Scientific Medical Center in Astana, Kazakhstan, between 2017 and 2022, and BM-MNCs were harvested from their anterior iliac crest. After isolating and cultivating the BM-MNCs, they were infused back into the patient's peripheral veins. Changes in BM-MNC and peripheral blood mononuclear cell (PB-MNC) phenotypes were assessed before and after a 24-hour cultivation period and 72 hours post-transplantation. We monitored liver function parameters over 6-month intervals and conducted flow cytometry analysis to assess CD markers on BM-MNCs before and after cultivation and PB-MNCs before and after transplantation. Statistical analysis included the Friedman test for liver parameters and the Wilcoxon signed-rank test for BM-MNC and PB-MNC comparisons.
Our findings revealed significant reductions in liver function tests after multiple transplantations. Flow cytometry analysis before and after a 24-hour culture and autologous BM-MNC infusion revealed the expansion of specific cell populations, with significant increases in CD3+, CD4+, CD16+, CD20+, CD25+, CD34+, CD105+, CD73+, СD117+, and CD34+populations, while CD4+25+, CD34+105+, and CD4+FOXP3+ populations decreased. Interestingly, a contradictory finding was observed with a decrease in bone marrow CD34+105+ cell lines (P=0.03) alongside an increase in peripheral CD34+105+ population (P=0.03).
In summary, our study shows that BM-MNC transplantation in PBC patients leads to changes in immune cell populations and liver function. These findings suggest potential therapeutic applications of BM-MNC transplantation in managing PBC and offer insights into the dynamics of immune cells associated with this treatment approach.
原发性胆汁性胆管炎（PBC）是一种影响肝脏和免疫系统的疾病。本研究旨在探讨自体骨髓源性单核细胞（BM-MNC）移植对 PBC 患者的影响。
2017 年至 2022 年期间，哈萨克斯坦阿斯塔纳国家科学医学中心的 16 名符合条件的 PBC 患者参与了该研究，并从其前髂嵴采集 BM-MNC。分离和培养 BM-MNC 后，将其输注回患者的外周静脉。在 24 小时培养期和移植后 72 小时前后评估 BM-MNC 和外周血单核细胞（PB-MNC）表型的变化。我们在 6 个月的时间间隔内监测肝功能参数，并进行流式细胞术分析，以评估培养前后 BM-MNC 和移植前后 PB-MNC 的 CD 标志物。统计分析包括 Friedman 检验用于肝功能参数，Wilcoxon 符号秩检验用于 BM-MNC 和 PB-MNC 的比较。
我们的研究结果表明，多次移植后肝功能检查显著降低。24 小时培养和自体 BM-MNC 输注前后的流式细胞术分析显示，特定细胞群的扩增，CD3+、CD4+、CD16+、CD20+、CD25+、CD34+、CD105+、CD73+、СD117+和 CD34+细胞群显著增加，而 CD4+25+、CD34+105+和 CD4+FOXP3+细胞群减少。有趣的是，观察到与骨髓 CD34+105+细胞系减少（P=0.03）相反的是，外周 CD34+105+群体增加（P=0.03）。
综上所述，我们的研究表明，BM-MNC 移植可导致 PBC 患者免疫细胞群和肝功能的变化。这些发现表明，BM-MNC 移植在治疗 PBC 方面具有潜在的治疗应用，并为这种治疗方法相关的免疫细胞动力学提供了新的见解。
