Nishikawa S, Morioka H, Tokunaga T, Aoyama Y, Kikyotani S, Fujimoto K, Yanase K, Tanaka T, Uesugi S, Ohtsuka E
Nucleic Acids Symp Ser. 1985(16):287-90.
RNase T1 gene and several mutant genes were constructed by joining of chemically synthesized deoxyoligonucleotides. These genes were inserted into an expression vector and expressed as fused protein in E. coli. RNase T1 and its mutant enzymes were liberated by cyanogen bromide treatment and their activities were measured.
