Ikehara M, Ohtsuka E, Uesugi S, Kikyodani T, Aoyama Y, Tokunaga T, Fujimoto K
Nucleic Acids Symp Ser. 1984(15):197-200.
In order to obtain knowledges about structure-function relationship of ribonuclease T1, we synthesized a structural gene for RNase T1 and several its modified genes. Using amino acid codons frequently used in Escherichia coli we designed genes consist of 328 X 2 bases. Synthesis of oligodeoxynucleotides with 9-20 base lengths was performed by 1% polystyrene supported triester approach and resulting 42 oligomers were joined together using T4 DNA ligase. The product was analyzed and utilyzed to construct expression vectors, which produced effectively fused proteins.
