• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于适体识别-释放策略的 SERS 分析平台用于结直肠癌前病变的高效灵敏诊断

SERS Analysis Platform Based on Aptamer Recognition-Release Strategy for Efficient and Sensitive Diagnosis of Colorectal Precancerous Lesions.

机构信息

Department of Gastroenterology, Nantong Haimen People's Hospital, Nantong, Jiangsu, 226100, People's Republic of China.

Department of gastroenterology, Traditional Chinese Medicine Hospital of Tongzhou District, Nantong, Jiangsu, 226300, People's Republic of China.

出版信息

Int J Nanomedicine. 2024 Sep 30;19:10009-10021. doi: 10.2147/IJN.S483261. eCollection 2024.

DOI:10.2147/IJN.S483261
PMID:39371477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11451456/
Abstract

BACKGROUND

Colorectal cancer (CRC) has become a significant global public health challenge, demanding immediate attention due to its high incidence and mortality rates. Regular CRC screening is essential for the early detection of precancerous lesions and CRC.

METHODS

: We developed a novel surface-enhanced Raman scattering (SERS) analysis platform that employs high-throughput microarray chips as carriers and Au/SnO nanoring arrays (Au/SnO NRAs) as substrates. This platform utilizes an aptamer recognition-release strategy to achieve efficient and sensitive detection of protein tumor markers. In the detection process, the strong affinity and high specificity between the aptamer and the target protein result in competitive replacement of the SERS nanoprobes originally bound to the substrate surface. As a result, the SERS nanoprobes carrying Raman reporter genes are dislodged, leading to a reduction in the SERS signal intensity.

RESULTS

The platform demonstrated excellent detection performance, with rapid detection completed within 15 minutes and limits of detection (LOD) as low as 6.2×10 g/mL for hnRNP A1 and 6.51×10 g/mL for S100P. Clinical samples analyzed using the SERS platform showed high consistency with enzyme-linked immunosorbent assay (ELISA) results.

CONCLUSION

This platform offers strong support for the early detection, risk assessment, and treatment monitoring of colorectal cancer precancerous lesions, with broad potential for clinical applications.

摘要

背景

结直肠癌（CRC）已成为一个重大的全球公共卫生挑战，由于其高发病率和死亡率，需要立即引起关注。定期进行 CRC 筛查对于早期发现癌前病变和 CRC 至关重要。

方法

我们开发了一种新型的表面增强拉曼散射（SERS）分析平台，该平台使用高通量微阵列芯片作为载体，金/锡氧化物纳米环阵列（Au/SnO NRAs）作为基底。该平台利用适体识别-释放策略实现对蛋白质肿瘤标志物的高效灵敏检测。在检测过程中，由于适体与目标蛋白之间具有很强的亲和力和高度特异性，导致最初结合在基底表面的 SERS 纳米探针发生竞争性取代。因此，携带拉曼报告基因的 SERS 纳米探针被洗脱，导致 SERS 信号强度降低。

结果

该平台表现出优异的检测性能，在 15 分钟内完成快速检测，检测限（LOD）对 hnRNP A1 低至 6.2×10 g/mL，对 S100P 低至 6.51×10 g/mL。使用 SERS 平台分析的临床样本与酶联免疫吸附测定（ELISA）结果高度一致。

结论

该平台为结直肠癌癌前病变的早期检测、风险评估和治疗监测提供了有力支持，具有广泛的临床应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/3808d9b23dea/IJN-19-10009-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/7b5a06ab4ec0/IJN-19-10009-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/810b936f7806/IJN-19-10009-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/479b579a8ef5/IJN-19-10009-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/bab7d17db2a3/IJN-19-10009-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/3338e6f3a211/IJN-19-10009-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/11cd6eafd091/IJN-19-10009-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/d94cbf5ad582/IJN-19-10009-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/3808d9b23dea/IJN-19-10009-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/7b5a06ab4ec0/IJN-19-10009-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/810b936f7806/IJN-19-10009-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/479b579a8ef5/IJN-19-10009-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/bab7d17db2a3/IJN-19-10009-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/3338e6f3a211/IJN-19-10009-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/11cd6eafd091/IJN-19-10009-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/d94cbf5ad582/IJN-19-10009-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3c0/11451456/3808d9b23dea/IJN-19-10009-g0008.jpg

相似文献

1
SERS Analysis Platform Based on Aptamer Recognition-Release Strategy for Efficient and Sensitive Diagnosis of Colorectal Precancerous Lesions.基于适体识别-释放策略的 SERS 分析平台用于结直肠癌前病变的高效灵敏诊断
Int J Nanomedicine. 2024 Sep 30;19:10009-10021. doi: 10.2147/IJN.S483261. eCollection 2024.
2
LoC-SERS platform for rapid and sensitive detection of colorectal cancer protein biomarkers.用于快速灵敏检测结直肠癌蛋白生物标志物的 LOC-SERS 平台。
Talanta. 2024 Apr 1;270:125563. doi: 10.1016/j.talanta.2023.125563. Epub 2023 Dec 19.
3
Aptamer-Based Functionalized SERS Biosensor for Rapid and Ultrasensitive Detection of Gastric Cancer-Related Biomarkers.基于适配体的功能化 SERS 生物传感器用于快速超灵敏检测胃癌相关生物标志物。
Int J Nanomedicine. 2023 Dec 11;18:7523-7532. doi: 10.2147/IJN.S434854. eCollection 2023.
4
Dual-recognition surface-enhanced Raman scattering(SERS)biosensor for pathogenic bacteria detection by using vancomycin-SERS tags and aptamer-FeO@Au.基于万古霉素 SERS 标签和适体-FeO@Au 的双识别表面增强拉曼散射(SERS)生物传感器用于致病菌检测。
Anal Chim Acta. 2019 Oct 24;1077:288-296. doi: 10.1016/j.aca.2019.05.059. Epub 2019 Jun 6.
5
Microfluidic-SERS sensing system based on dual signal amplification and aptamer for gastric cancer detection.基于双重信号放大和适体的用于胃癌检测的微流控 SERS 传感系统。
Mikrochim Acta. 2024 Oct 14;191(11):668. doi: 10.1007/s00604-024-06760-z.
6
Indirect surface-enhanced Raman scattering assay of insulin-like growth factor 2 receptor protein by combining the aptamer modified gold substrate and silver nanoprobes.通过将适配体修饰的金基底与银纳米探针相结合，实现对胰岛素样生长因子 2 受体蛋白的间接表面增强 Raman 散射检测。
Mikrochim Acta. 2020 Feb 10;187(3):160. doi: 10.1007/s00604-020-4126-x.
7
Sensitivity-improved SERS detection of SARS-CoV-2 spike protein by Au NPs/COFs integrated with catalytic-hairpin-assembly amplification technology.通过 Au NPs/COFs 与催化发夹组装扩增技术集成，提高了对 SARS-CoV-2 刺突蛋白的 SERS 检测灵敏度。
Anal Chim Acta. 2024 Aug 22;1318:342924. doi: 10.1016/j.aca.2024.342924. Epub 2024 Jun 29.
8
Ultrasensitive detection of SARS-CoV-2 S protein with aptamers biosensor based on surface-enhanced Raman scattering.基于表面增强拉曼散射的适体生物传感器超灵敏检测 SARS-CoV-2 S 蛋白。
J Chem Phys. 2023 Jan 14;158(2):024203. doi: 10.1063/5.0130011.
9
Ultrasensitive detection of plant hormone abscisic acid-based surface-enhanced Raman spectroscopy aptamer sensor.基于超敏检测植物激素脱落酸的表面增强拉曼光谱适体传感器。
Anal Bioanal Chem. 2022 Mar;414(8):2757-2766. doi: 10.1007/s00216-022-03923-w. Epub 2022 Feb 10.
10
Surface-enhanced Raman scatting microfluidic chip based on the identification competition strategy were used for rapid and simultaneous detection of liver cancer related proteins.基于识别竞争策略的表面增强 Raman 散射微流控芯片用于快速同时检测肝癌相关蛋白。
Photodiagnosis Photodyn Ther. 2024 Oct;49:104336. doi: 10.1016/j.pdpdt.2024.104336. Epub 2024 Sep 19.

引用本文的文献

1
Aptamer and Oligonucleotide-Based Biosensors for Health Applications.用于健康应用的适配体和基于寡核苷酸的生物传感器。
Biosensors (Basel). 2025 Apr 29;15(5):277. doi: 10.3390/bios15050277.
2
Multiple roles of S100P in pan carcinoma: Biological functions and mechanisms (Review).S100P在泛癌中的多重作用：生物学功能与机制（综述）
Oncol Rep. 2025 Jun;53(6). doi: 10.3892/or.2025.8895. Epub 2025 Apr 11.

本文引用的文献

1
SERS-based microdevices for use as diagnostic biosensors.基于 SERS 的微器件，用作诊断生物传感器。
Chem Soc Rev. 2024 Jun 4;53(11):5394-5427. doi: 10.1039/d3cs01055d.
2
Enhancement Factors: A Central Concept during 50 Years of Surface-Enhanced Raman Spectroscopy.增强因子：表面增强拉曼光谱50年历程中的核心概念。
ACS Nano. 2024 Apr 9;18(14):9773-9783. doi: 10.1021/acsnano.4c01474. Epub 2024 Mar 26.
3
Colon and rectal cancer: An emergent public health problem.结直肠癌：一个亟待解决的公共卫生问题。
World J Gastroenterol. 2024 Feb 21;30(7):644-651. doi: 10.3748/wjg.v30.i7.644.
4
Noble metal-free SERS: mechanisms and applications.无贵金属表面增强拉曼光谱：机理与应用
Analyst. 2023 Dec 18;149(1):11-28. doi: 10.1039/d3an01669b.
5
Application of artificial intelligence in colorectal cancer screening by colonoscopy: Future prospects (Review).人工智能在结肠镜结直肠癌筛查中的应用：未来展望（综述）。
Oncol Rep. 2023 Nov;50(5). doi: 10.3892/or.2023.8636. Epub 2023 Sep 29.
6
Plasma protein changes reflect colorectal cancer development and associated inflammation.血浆蛋白变化反映结直肠癌的发展及相关炎症。
Front Oncol. 2023 May 9;13:1158261. doi: 10.3389/fonc.2023.1158261. eCollection 2023.
7
The Diet as a Modulator of Tumor Microenvironment in Colorectal Cancer Patients.饮食作为结直肠癌患者肿瘤微环境的调节剂。
Int J Mol Sci. 2023 Apr 15;24(8):7317. doi: 10.3390/ijms24087317.
8
Novel DNA methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions.粪便和血液中新的 DNA 甲基化生物标志物用于结直肠癌和癌前病变的早期检测。
Clin Epigenetics. 2023 Feb 17;15(1):26. doi: 10.1186/s13148-023-01443-7.
9
Immunohistochemistry and Immunofluorescence.免疫组织化学与免疫荧光
Methods Mol Biol. 2023;2588:439-450. doi: 10.1007/978-1-0716-2780-8_26.
10
FJX1 as a candidate diagnostic and prognostic serum biomarker for colorectal cancer.FJX1 作为结直肠癌的候选诊断和预后血清生物标志物。
Clin Transl Oncol. 2022 Oct;24(10):1964-1974. doi: 10.1007/s12094-022-02852-5. Epub 2022 Jun 1.