Department of Gastrointestinal Surgery, Jiangdu People's Hospital Affiliated to Yangzhou University, Yangzhou, 225200, P. R. China.
Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, 225001, P. R. China.
Mikrochim Acta. 2024 Oct 14;191(11):668. doi: 10.1007/s00604-024-06760-z.
Studies have found that matrix metalloproteinase-9 (MMP-9) and interleukin-6 (IL-6) play an important role in tumorigenesis. In order to detect MMP-9 and IL-6 concentrations with high sensitivity and specificity, an efficient microfluidic-SERS sensing system was prepared based on surface-enhanced Raman scattering (SERS). The aptamer recognition-release mechanism and the dual signal amplification strategy were applied in the sensing system. The sensor system was developed using two kinds of nanomaterials with excellent SERS properties, namely gold-coated iron tetroxide particles (FeO@AuNPs) and gold nanocages (AuNCs). In addition, FeO@AuNPs also has magnetic adsorption properties. In the sensing system, single-stranded DNA1 (ssDNA1) and aptamer were modified on FeO@AuNPs. Single-stranded DNA2 (ssDNA2) and Raman tags were modified on AuNCs. When the target was present, the aptamer bound to the target and detached from the FeO@AuNPs, and ssDNA2 bound to the exposed ssDNA1. At this time, the FeO@AuNPs@AuNCs@SERS tag complex was formed, and the SERS signal was enhanced for the first time. Under the action of an external magnet on the microfluidic chip, the complex was magnetized and enriched. The SERS signal was enhanced for the second time. Due to the high affinity between the aptamer and the target object, the sensing system has a strong specificity. The double amplification of the SERS signal gave the system excellent sensitivity. The limit of detection (LOD) relative to MMP-9 and IL-6 were as low as 0.178 pg/mL and 0.165 pg/mL, respectively. The microfluidic-SERS sensing system has a feasible prospect in the early screening of gastric cancer.
研究发现基质金属蛋白酶-9（MMP-9）和白细胞介素-6（IL-6）在肿瘤发生中起着重要作用。为了高灵敏度和特异性地检测 MMP-9 和 IL-6 浓度，我们基于表面增强拉曼散射（SERS）制备了一种高效的微流控-SERS 传感系统。该传感系统应用了适体识别-释放机制和双信号放大策略。该传感器系统采用了两种具有优异 SERS 性能的纳米材料，即金包覆四氧化三铁颗粒（FeO@AuNPs）和金纳米笼（AuNCs）。此外，FeO@AuNPs 还具有磁性吸附性能。在传感系统中，单链 DNA1（ssDNA1）和适体被修饰在 FeO@AuNPs 上。ssDNA2 和拉曼标记被修饰在 AuNCs 上。当存在靶标时，适体与靶标结合并从 FeO@AuNPs 上脱离，ssDNA2 与暴露的 ssDNA1 结合。此时，形成了 FeO@AuNPs@AuNCs@SERS 标记物复合物，SERS 信号得到了第一次增强。在外加磁场的作用下，复合物在微流控芯片上被磁化和富集，SERS 信号得到了第二次增强。由于适体与靶标之间的高亲和力，该传感系统具有很强的特异性。SERS 信号的双重放大赋予了系统优异的灵敏度。相对于 MMP-9 和 IL-6 的检测限（LOD）分别低至 0.178 pg/mL 和 0.165 pg/mL。微流控-SERS 传感系统在胃癌的早期筛查中具有可行的前景。
