Park Se Won, Lee Hui Jeong, Kim Jimi, Kim Tae Yang, Seo Young Rok, Kim Gwang Il, Cho Sungeun, Kim Mi Jeong
Interdisciplinary Program in Senior Human Ecology, Changwon National University, Gyeongnam 51140, Korea.
Department of Food and Nutrition, Changwon National University, Gyeongnam 51140, Korea.
Prev Nutr Food Sci. 2024 Sep 30;29(3):376-383. doi: 10.3746/pnf.2024.29.3.376.
This study investigated the antioxidative characteristics of L. purple corn cob and husk extract (PCHE) and its potential protective effects against blue light (BL)-induced damage in N-retinylidene-N-retinylethanolamine (A2E)-accumulated ARPE-19 retinal pigment epithelial cells. PCHE had a 2,2-diphenyl-1-picrylhydrazyl radical-scavenging capacity and Trolox equivalent antioxidant capacity of 1.28±0.43 mM Trolox equivalents (TE)/g and 2,545.41±34.13 mM TE/g, respectively. Total content of anthocyanins, polyphenols, and flavonoids in the PCHE was 11.13±0.10 mg cyanidin-3-glucoside equivalents/100 g, 227.90±7.38 mg gallic acid equivalents/g, and 117.75±2.46 mg catechin equivalents/g, respectively. PCHE suppressed the accumulation of A2E and the photooxidation caused by BL in a dose-dependent manner. After initial treatment with 25 µM/mL A2E and BL, ARPE-19 cells showed increased cell viability following additional treatment with 15 µg/mL PCHE while the expression of the p62 sequestosome 1 decreased, whereas that of heme oxygenase-1 protein increased compared with that in cells without PCHE treatment. This suggests that PCHE may slow the autophagy induced by BL exposure in A2E-accumulated retinal cells and protect them against oxidative stress.
本研究调查了紫玉米芯和苞叶提取物（PCHE）的抗氧化特性及其对蓝光（BL）诱导的N - 视黄叉 - N - 视黄基乙醇胺（A2E）积累的ARPE - 19视网膜色素上皮细胞损伤的潜在保护作用。PCHE的2,2 - 二苯基 - 1 - 苦基肼自由基清除能力和Trolox等效抗氧化能力分别为1.28±0.43 mM Trolox当量（TE）/g和2545.41±34.13 mM TE/g。PCHE中花青素、多酚和黄酮类化合物的总含量分别为11.13±0.10 mg矢车菊素 - 3 - 葡萄糖苷当量/100 g、227.90±7.38 mg没食子酸当量/g和117.75±2.46 mg儿茶素当量/g。PCHE以剂量依赖性方式抑制A2E的积累和BL引起的光氧化。在用25 µM/mL A2E和BL进行初始处理后，与未用PCHE处理的细胞相比，用15 µg/mL PCHE进行额外处理后，ARPE - 19细胞的细胞活力增加，而p62蛋白（p62 sequestosome 1）的表达降低，血红素加氧酶 - 1蛋白的表达增加。这表明PCHE可能减缓BL暴露在A2E积累的视网膜细胞中诱导的自噬，并保护它们免受氧化应激。
