Lee Ju Eun, Noh Sang-Kyu, Kim Mi Jeong
Interdisciplinary Program in Senior Human Ecology, Changwon National University, Changwon 51140, Korea.
Department of Food and Nutrition, Changwon National University, Changwon 51140, Korea.
Antioxidants (Basel). 2022 Oct 26;11(11):2112. doi: 10.3390/antiox11112112.
Collagen hydrolysate were extracted from Alaska pollock skin using enzymatic (EAE), ultrasound (UAE), or combination of enzymatic and ultrasound (EAE+UAE) treatment. Control (C) was not treated with enzymatic or ultrasound. The extracts from C, EAE, UAE, and EAE+UAE were fractionated with ≤3, 3-10, 10-30, and ≥30 kDa. Each fraction was evaluated for biological activity and structural properties. All fractions contained high levels of glycine and proline. The ≤3 kDa fraction of control and ultrasound-assisted extracts exhibited the highest antioxidant activity as measured using Trolox equivalent antioxidant capacity, ferric ion reducing antioxidant power, oxygen radical absorbance capacity, and an assay on the inhibition of nitric oxide production by LPS-induced macrophages. The structurally digested collagen was evaluated using FTIR spectra and SDS-PAGE after Alcalase and ultrasound treatments. The microstructure of collagen hydrolysate was assessed using SEM microscopy; the surface morphology was altered according to fraction size and extraction conditions. Overall, it was determined that enzyme treatment in combination with ultrasound is the most effective procedure for obtaining digested collagen hydrolysate, which could be used to further improve biotechnological processing for the addition of value to marine production chains in the future.
采用酶法（EAE）、超声法（UAE）或酶法与超声联合法（EAE+UAE）从狭鳕鱼皮中提取胶原蛋白水解物。对照组（C）未进行酶法或超声处理。将C、EAE、UAE和EAE+UAE的提取物按分子量≤3 kDa、3-10 kDa、10-30 kDa和≥30 kDa进行分级分离。对每个级分的生物活性和结构特性进行评估。所有级分均含有高水平的甘氨酸和脯氨酸。对照组和超声辅助提取物的≤3 kDa级分表现出最高的抗氧化活性，采用Trolox等效抗氧化能力、铁离子还原抗氧化能力、氧自由基吸收能力以及脂多糖诱导巨噬细胞产生一氧化氮的抑制试验进行测定。在碱性蛋白酶和超声处理后，使用傅里叶变换红外光谱（FTIR）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对结构消化的胶原蛋白进行评估。使用扫描电子显微镜（SEM）对胶原蛋白水解物的微观结构进行评估；表面形态根据级分大小和提取条件而改变。总体而言，确定酶法与超声联合处理是获得消化胶原蛋白水解物的最有效方法，未来可用于进一步改进生物技术加工，为海洋生产链增加价值。
