Pattiyakumbura T T, Malkanthi K G K, Dheerasekara W K H, Manamperi A, Muthugala M A R V
National Hospital, Kandy 20000, Sri Lanka.
Faculty of Allied Health Sciences, University of Peradeniya, Peradeniya 20400, Sri Lanka.
Access Microbiol. 2024 Oct 3;6(10). doi: 10.1099/acmi.0.000838.v3. eCollection 2024.
Hepatitis B infection causes a spectrum of clinical diseases varying from asymptomatic infection to severe or fulminant acute hepatitis, chronic liver disease, cirrhosis and hepatocellular carcinoma. Hepatitis B virus (HBV) genotypes appear to influence transmission dynamics, clinical outcomes and responses to antiviral therapy. However, hepatitis B genotyping has been poorly investigated in Sri Lanka. This study intended to determine hepatitis B genotypes in a group of HBV-infected people in central and northern Sri Lanka. The study was a laboratory-based descriptive cross-sectional study. Initial detection of HBV DNA in 100 EDTA blood samples was done by using a commercially validated quantitative real-time PCR kit. Hepatitis B genotyping was performed by in-house conventional semi-nested multiplex PCR using genotype-specific primers (for genotypes A-F). The serological profile was determined using a commercially validated ELISA/chemiluminescence immunoassay. The results were evaluated for genotype prevalence, viral load association and hepatitis B e antigen (HBeAg) expression in the study population. The study detected that genotype C (=38) is most prevalent and infections with multiple genotypes (=52, 52%) were commoner than mono-genotype (=23, 23%) infections. In total, 25% of patients had no detectable genotype among genotypes A-F. The mean viral load in asymptomatic patients with a single genotype was 3.28 log copies ml and in multiple genotypes was 4.18 log copies ml before treatment. Statistical significance was not detected in mean viral loads and HBeAg expression in these two groups. In the future, chronic HBV infection may be effectively treated and managed according to the infected genotype.
乙型肝炎感染可导致一系列临床疾病，从无症状感染到严重或暴发性急性肝炎、慢性肝病、肝硬化和肝细胞癌。乙型肝炎病毒（HBV）基因型似乎会影响传播动态、临床结局以及对抗病毒治疗的反应。然而，斯里兰卡对乙型肝炎基因分型的研究较少。本研究旨在确定斯里兰卡中部和北部一组HBV感染者的乙型肝炎基因型。该研究是一项基于实验室的描述性横断面研究。使用经过商业验证的定量实时PCR试剂盒对100份EDTA血样中的HBV DNA进行初步检测。通过使用基因型特异性引物（针对A - F基因型）的内部常规半巢式多重PCR进行乙型肝炎基因分型。使用经过商业验证的ELISA/化学发光免疫测定法确定血清学特征。对研究人群中的基因型流行率、病毒载量关联和乙型肝炎e抗原（HBeAg）表达结果进行评估。研究发现基因型C（=38）最为常见，多重基因型感染（=52，52%）比单一基因型感染（=23，23%）更常见。总共有25%的患者在A - F基因型中未检测到基因型。治疗前，单一基因型无症状患者的平均病毒载量为3.28 log拷贝/毫升，多重基因型患者为4.18 log拷贝/毫升。这两组的平均病毒载量和HBeAg表达未检测到统计学意义。未来，慢性HBV感染可能根据感染的基因型进行有效治疗和管理。
