Dahlan Nelly Herfina, Sitohang Irma Bernadette S, Indriatmi Wresti, Wibowo Heri, Enggy Liani Elisabeth
Department of Dermatology and Venereology, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Department of Integrated Laboratory, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Clin Cosmet Investig Dermatol. 2024 Oct 1;17:2183-2195. doi: 10.2147/CCID.S475068. eCollection 2024.
The inflammatory process in acne vulgaris (AV) is characterized by the upregulation of specific pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6, and IL-8, within sebocytes and keratinocytes. Sebocytes have been identified as target cells for bioactive vitamin D. Experimental studies on animal models have demonstrated the potent comedolytic effects of topical vitamin D. However, further research is required to specifically evaluate the impact of vitamin D on inflammatory lesions in acne vulgaris (AV).
To evaluate the effectiveness of topical vitamin D in treating acne vulgaris (AV) lesions by investigating its anti-inflammatory effects on pro-inflammatory cytokine modulation, specifically assessing the correlation between IL-1β levels in acne lesions and the reduction in AV severity.
This study is a double-blind, randomized, placebo-controlled clinical trial with a 2-arm design over an 8-week intervention period. Participants were randomly assigned to either the topical vitamin D group (cholecalciferol 50 mcg) or the topical placebo group, with each group comprising 32 subjects. All participants received concomitant treatment with topical adapalene 0.1%. Cytokine levels within acne lesions were assessed using Luminex Polystyrene Screening Assays to detect and quantify IL-1β levels. The effectiveness of the treatment was evaluated by monitoring the reduction in the number of inflammatory lesions, while the safety of topical vitamin D was assessed by documenting and analyzing any reported side effects.
The study found a significant correlation between the reduction in IL-1β levels within acne lesions and the decrease in moderate and severe inflammatory lesions in acne vulgaris (p = 0.028). The topical application of vitamin D led to a significant reduction in inflammatory AV lesions (p = 0.045). No significant topical side effects were observed in either the vitamin D or placebo groups.
This study demonstrates that the topical administration of vitamin D in acne vulgaris (AV) lesions is effective in reducing pro-inflammatory cytokine levels within acne lesions and in decreasing the severity of AV.
NCT05758259. September 5, 2022.
寻常痤疮（AV）中的炎症过程的特征是皮脂腺细胞和角质形成细胞内特定促炎细胞因子的上调，包括白细胞介素（IL）-1β、IL-6和IL-8。皮脂腺细胞已被确定为生物活性维生素D的靶细胞。对动物模型的实验研究表明局部应用维生素D具有强大的抗粉刺作用。然而，需要进一步研究以具体评估维生素D对寻常痤疮（AV）炎症性皮损的影响。
通过研究局部应用维生素D对促炎细胞因子调节的抗炎作用，特别是评估痤疮皮损中IL-1β水平与AV严重程度降低之间的相关性，来评估局部应用维生素D治疗寻常痤疮（AV）皮损的有效性。
本研究是一项双盲、随机、安慰剂对照的临床试验，采用双臂设计，干预期为8周。参与者被随机分配到局部应用维生素D组（胆钙化醇50微克）或局部应用安慰剂组，每组32名受试者。所有参与者均接受0.1%局部用阿达帕林的联合治疗。使用Luminex聚苯乙烯筛选试验评估痤疮皮损内的细胞因子水平，以检测和量化IL-1β水平。通过监测炎症性皮损数量的减少来评估治疗效果，同时通过记录和分析任何报告的副作用来评估局部应用维生素D的安全性。
研究发现痤疮皮损内IL-1β水平的降低与寻常痤疮中重度炎症性皮损的减少之间存在显著相关性（p = 0.028）。局部应用维生素D导致炎症性AV皮损显著减少（p = 0.045）。维生素D组和安慰剂组均未观察到明显的局部副作用。
本研究表明，在寻常痤疮（AV）皮损中局部应用维生素D可有效降低痤疮皮损内促炎细胞因子水平，并降低AV的严重程度。
NCT05758259。2022年9月5日。
