Wang Cen, Hou Lilong, Jiang Nan, Wang Yu, Mao Xiaofen, Zhou Ping, Xia Yin, Wang Yuanyuan, Chen Chuyue, Yang Xinyu, Luo Qiang, Pan Jiao
Key Laboratory of Archaeomaterials and Conservation, Ministry of Education, Institute for Cultural Heritage and History of Science and Technology, University of Science and Technology Beijing, Beijing, China.
Ministry of Education Key Laboratory of Molecular Microbiology and Technology, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.
Front Microbiol. 2024 Sep 20;15:1378180. doi: 10.3389/fmicb.2024.1378180. eCollection 2024.
Earthen sites are essential cultural relic resources, and site museums are a fundamental component of China's cultural heritage protection. The mausoleum of the Qin Shi Huang Emperor is one of the largest, most peculiar, and richest imperial tombs in the world. The exhibition hall of the burial pit No. 1 of the Terra Cotta Warriors is the earliest exhibition hall built and opened to the public. However, after years of excavation and open exhibitions, the earthen site of the Emperor Qinshihuang's Mausoleum Site Museum has deteriorated to varying degrees due to changes in the modern environment. There is an urgent need to control microbial diseases and protect the earthen site.
We analyzed the physical and chemical properties and bioindicators of the collected soil samples. We also established a metagenomic library and conducted a correlation analysis between microbial community composition and environmental factors. Cultivable fungi obtained from air and soil samples were identified, and allicin volatile gas fungistasis test was conducted.
Research has found that four different areas of the exhibition hall have different types of microbial diseases owing to their different environments. The main pathogenic fungi in earthen site may lead to potential microbial diseases that affect important cultural relics such as the Terra Cotta Warriors. , and showed relatively specific growth in relation to environmental factors and showed a better raw growth advantage.Allicin gas had a inhibitory effect on 12 types of fungi, therefore allicin gas had a potent inhibitory effect on the growth of the most culturable fungal hyphae.
This study provides basic data for the study of microbial diversity in the exhibition hall of Pit No. 1 at the Terracotta Warriors Museum in Emperor Qinshihuang's Mausoleum Site Museum. It provides a reference for future protection work, which is of great significance.
土遗址是重要的文物资源,遗址博物馆是中国文化遗产保护的重要组成部分。秦始皇陵是世界上规模最大、形制最奇特、内涵最丰富的帝王陵墓之一。兵马俑一号坑展厅是最早建成并向公众开放的展厅。然而,经过多年的发掘和开放展览,秦始皇陵兵马俑博物馆土遗址因现代环境变化而出现不同程度的劣化。迫切需要控制微生物病害,保护土遗址。
分析采集土壤样本的理化性质和生物指标。建立宏基因组文库,进行微生物群落组成与环境因子的相关性分析。对空气和土壤样本中可培养真菌进行鉴定,并进行大蒜素挥发性气体抑菌试验。
研究发现,展厅四个不同区域因环境不同而存在不同类型的微生物病害。土遗址中的主要致病真菌可能引发潜在的微生物病害,影响兵马俑等重要文物。 、 和 相对于环境因子表现出相对特异性的生长,并表现出较好的原生生长优势。大蒜素气体对12种真菌有抑制作用,因此大蒜素气体对大多数可培养真菌菌丝的生长有较强的抑制作用。
本研究为秦始皇陵兵马俑博物馆一号坑展厅微生物多样性研究提供了基础数据,为今后的保护工作提供了参考,具有重要意义。