Karthik Kumaragurubaran, Anbazhagan Subbaiyan, Priyadharshini Murugaiyan Latha Mala, Sharma Rajeev Kumar, Manoharan Seeralan
Veterinary College and Research Institute, Udumalpet, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051 India.
ICMR-National Animal Resource Facility for Biomedical Research, Hyderabad, 500078 India.
3 Biotech. 2024 Nov;14(11):257. doi: 10.1007/s13205-024-04102-7. Epub 2024 Oct 4.
, a zoonotic pathogen causing enteric diseases in different animals and humans. A comprehensive study on the presence of toxin genes and antimicrobial resistance genes based on genome data of in animals is scanty. In the present study, a total of 15 isolates were recovered from dogs and isolates with toxin genes (D1, CD15 and CD26) along with two other non-toxigenic strains (CD28, CD32) were used for whole genome sequencing and comparative genomics. Sequence type-based clustering was noted in the whole genome phylogeny with 4 known multi-locus sequence typing (MLST) clades namely I, II, IV, and V and a cryptic clade. ST11 and ST54 were reported for the 2 time worldwide in dogs. Out of 109 genomes used in the study, 29 genomes were predicted with all four toxin genes (, , , ) while 22 did not have any of the toxin genes. ST11 of MLST clade V had the maximum number of 46 genomes predicted with at least one toxin gene. Among the genomes sequenced in this study, CD26 had a maximum of 5 AMR genes (, , , , and ) and CD15 was predicted with 2 AMR genes (, ). Tetracycline resistance genes were predicted most in the ST11 genome. Of the 22 non-toxigenic strains, 9 genomes (ST48 = 5, ST3 = 2, ST109 = 1, ST15 = 1) were predicted with a minimum of one AMR gene. Pangenome analysis indicated that the value is 0.12 showing that has an open pangenome structure. This indicates that the organism can evolve by the addition of new genes. This study reports the circulation of clinically important ST11 and multidrug-resistant non-toxigenic strains among animals.
The online version contains supplementary material available at 10.1007/s13205-024-04102-7.
是一种人畜共患病原体，可在不同动物和人类中引起肠道疾病。基于动物基因组数据对毒素基因和抗菌药物耐药基因存在情况的全面研究较少。在本研究中，共从狗身上分离出15株菌株，将带有毒素基因（D1、CD15和CD26）的分离株以及另外两株无毒菌株（CD28、CD32）用于全基因组测序和比较基因组学研究。在全基因组系统发育中发现了基于序列类型的聚类，有4个已知的多位点序列分型（MLST）分支，即I、II、IV和V以及一个隐性分支。ST11和ST54在全球范围内是第二次在狗身上被报道。在该研究使用的109个基因组中，29个基因组被预测含有所有4种毒素基因（、、、），而22个基因组没有任何毒素基因。MLST分支V的ST11预测至少含有一种毒素基因的基因组数量最多，为46个。在本研究测序的基因组中，CD26最多有5个抗菌药物耐药基因（、、、、），CD15被预测有2个抗菌药物耐药基因（、）。四环素耐药基因在ST11基因组中预测最多。在22株无毒菌株中，9个基因组（ST48 = 5、ST3 = 2、ST109 = 1、ST15 = 1）被预测至少含有一个抗菌药物耐药基因。泛基因组分析表明值为0.12，表明具有开放的泛基因组结构。这表明该生物体可以通过添加新基因而进化。本研究报告了临床上重要的ST11和多药耐药无毒菌株在动物中的传播情况。
在线版本包含可在10.1007/s13205-024-04102-7获取的补充材料。
