Panda Bandana, Patri Gaurav, Jena Debkant, Behera Ranjan Rashmi, Agarwal Vanshika, Gupta Aditi
Department of Conservative Dentistry and Endodontics, Kalinga Institute of Dental Sciences, Bhubaneswar, Odisha, India.
Department of Periodontics and Oral Implantology, Kalinga Institute of Dental Sciences, Bhubaneswar, Odisha, India.
J Conserv Dent Endod. 2024 Aug;27(8):853-859. doi: 10.4103/JCDE.JCDE_320_24. Epub 2024 Aug 7.
Clinical evidence of platelet-rich fibrin (PRF) benefits on bone repair is still emerging, prompting researchers to experiment with different PRF formulations as osteoconductive scaffolds.
This study compared the osteoconductive effects of injectable PRF (i-PRF) and leukocyte-rich PRF (L-PRF) on the differentiation of dental pulp stem cells (DPSCs) into osteoblasts.
Blood samples were collected from the volunteers to prepare L-PRF and i-PRF conditioned media (CM) by centrifugation. DPSCs were isolated from impacted third molars and cultured. Proliferation of DPSCs in response to L-PRF and i-PRF was assessed by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Osteoinductive potential was evaluated through alkaline phosphatase (ALP) activity, alizarin red S (ARS) staining, growth factor levels (vascular endothelial growth factor [VEGF], transforming growth factor [TGF-beta]), and cytokine expression (interleukin 6 [IL-6], IL-8) after 7 days.
MTT assay results showed that both L-PRF and i-PRF increased DPSC proliferation relative to the control group. After 7 days in L-PRF and i-PRF CM, DPSCs exhibited increased ALP activity, higher red-colored calcium deposits with ARS staining, and elevated levels of VEGF and TGF-beta. In addition, higher concentrations of inflammatory cytokines IL-6 and IL-8 were observed in both L-PRF and i-PRF compared to the control.
Using both L-PRF and i-PRF as scaffolds can enhance the osteoinductive ability of stem cells, offering a potential strategy for regenerative therapies.
富含血小板纤维蛋白（PRF）对骨修复有益的临床证据仍在不断涌现，这促使研究人员尝试将不同的PRF配方用作骨传导支架。
本研究比较了可注射PRF（i-PRF）和富含白细胞PRF（L-PRF）对牙髓干细胞（DPSC）向成骨细胞分化的骨传导作用。
从志愿者身上采集血液样本，通过离心制备L-PRF和i-PRF条件培养基（CM）。从阻生第三磨牙中分离并培养DPSC。通过MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）试验评估DPSC对L-PRF和i-PRF的增殖反应。7天后，通过碱性磷酸酶（ALP）活性、茜素红S（ARS）染色、生长因子水平（血管内皮生长因子[VEGF]、转化生长因子[TGF-β]）和细胞因子表达（白细胞介素6[IL-6]、IL-8）评估骨诱导潜力。
MTT试验结果表明，与对照组相比，L-PRF和i-PRF均增加了DPSC的增殖。在L-PRF和i-PRF CM中培养7天后，DPSC表现出ALP活性增加、ARS染色显示红色钙沉积增多以及VEGF和TGF-β水平升高。此外，与对照组相比，在L-PRF和i-PRF中均观察到更高浓度的炎性细胞因子IL-6和IL-8。
将L-PRF和i-PRF用作支架均可增强干细胞的骨诱导能力，为再生治疗提供了一种潜在策略。
