Private Denizli Tekden Hospital, Denizli, Türkiye.
Kütahya Dumlupınar University Faculty of Arts and Sciences, Department of Molecular Biology; Zoonoses Application and Research Center, Kütahya, Türkiye.
Turkiye Parazitol Derg. 2024 Oct 7;48(3):184-190. doi: 10.4274/tpd.galenos.2024.60392.
This study was conducted to molecularly identify and classify spp. in fecal samples (n=150) from patients with diarrhea received at the microbiology laboratory of a private hospital in Denizli.
In this study, the positivity of spp. in fecal samples was investigated using direct microscopy, Kinyoun's acid-fast staining method, and Nested polymerase chain reaction (PCR) techniques. Positive PCR products were sequenced.
In the examined fecal samples of patients with diarrhea, no parasites were detected through direct microscopic examination. Using the Kinyoun acid-fast staining method, spp. was identified in 2.7% (n=4) of the samples, while Nested PCR detected it in 4.67% (n=7) of the samples. The four positive samples were sequenced using primers that amplify the 18S gene region. The sequencing results identified the isolates as .
Cryptosporidiosis is an important public health issue as it is a zoonotic disease caused by the parasite that can be transmitted from animals to humans. This study focuses on the molecular characterization of species detected in human fecal samples, which is significant for understanding which specific strains or species are involved in human infections. According to the findings, it is recommended that control measures be implemented to reduce the risk of exposure to in both humans and animals in Türkiye.
本研究旨在通过分子生物学方法对来自代尼兹利一家私立医院微生物实验室的腹泻患者粪便样本（n=150）中的 spp.进行鉴定和分类。
本研究采用直接显微镜检查、金尼翁抗酸染色法和巢式聚合酶链反应（PCR）技术检测粪便样本中 spp.的阳性率。对阳性 PCR 产物进行测序。
在检查的腹泻患者粪便样本中，直接显微镜检查未发现寄生虫。金尼翁抗酸染色法鉴定出 2.7%（n=4）的样本中存在 spp.，而巢式 PCR 检测出 4.67%（n=7）的样本中存在该菌。用扩增 18S rRNA 基因区域的引物对四个阳性样本进行测序。测序结果鉴定出分离株为.
隐孢子虫病是一个重要的公共卫生问题，因为它是一种由寄生虫引起的人畜共患病，可以从动物传播给人类。本研究专注于检测到的人类粪便样本中 种的分子特征，这对于了解哪些特定的菌株或种参与人类感染非常重要。根据研究结果，建议在土耳其实施控制措施，以减少人类和动物暴露于 的风险。
