Zhao Rui, Wang Huan-Huan, Wang Zhuo, Xiao Xing, Yin Xin-Hui, Hu Shi-Yuan, Miao Hao-Nan, Zhang Yong-Jun, Liang Pei, Gu Shao-Hua
Department of Entomology, China Agricultural University, Beijing 100193, China.
Sanya Institute of China Agricultural University, Sanya 572024, China.
J Agric Food Chem. 2024 Oct 7. doi: 10.1021/acs.jafc.4c05737.
is a notorious pest that develops a high resistance to many insecticides. Recently, insect odorant-binding proteins (OBPs) have been proven to participate in insecticide resistance. However, the functional evidence supporting the cross-link between OBPs and insecticide resistance remains unexplored. Here, we identified 50 SfruOBPs from the larval transcriptome and genome. Notably, was highly expressed in the larval cuticle and could be induced to upregulate its expression by multi-insecticides. Ligand-binding assays revealed that bound strongly with four insecticides; RNAi and insecticide bioassay demonstrated that the knockdown of did not affect larval survival and development. However, it can significantly increase the larval susceptibility to multi-insecticides, suggesting an uncommon role of in multi-insecticide susceptibility. Our study provides a comprehensive understanding of SfruOBPs and furthermore proves that a larval cuticle-enriched OBP can bind with and confer larval tolerance to multi-insecticides. could be a new insecticidal target for controlling Lepidoptera pests.
是一种臭名昭著的害虫，对许多杀虫剂产生了高度抗性。最近，昆虫气味结合蛋白（OBPs）已被证明参与了抗药性。然而，支持OBPs与抗药性之间交联的功能证据仍未得到探索。在这里，我们从幼虫转录组和基因组中鉴定出50种SfruOBPs。值得注意的是，在幼虫表皮中高度表达，并且可以被多种杀虫剂诱导上调其表达。配体结合试验表明，与四种杀虫剂强烈结合；RNA干扰和杀虫剂生物测定表明，敲低不会影响幼虫的存活和发育。然而，它可以显著增加幼虫对多种杀虫剂的敏感性，表明在多种杀虫剂敏感性中具有不同寻常的作用。我们的研究提供了对SfruOBPs的全面理解，并且进一步证明，一种富含幼虫表皮的OBP可以与多种杀虫剂结合并赋予幼虫耐受性。可能是控制鳞翅目害虫的一个新的杀虫靶点。
