Chen Lei, Guo Li-Xue, Zhang Hua-Meng, Sun Xiao-Nan, Liu Yu-Ting, Ji Jie, Xue Wen-Xin, Wybouw Nicky, Van Leeuwen Thomas, Xue Xiao-Feng, Hong Xiao-Yue, Sun Jing-Tao
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Commun Biol. 2025 Aug 12;8(1):1197. doi: 10.1038/s42003-025-08658-9.
The rapid evolution of pesticide resistance imposes great pressure on food production. However, how resistance alleles arise and spread across field populations remains largely understood. Here, we study the evolutionary trajectories of resistance alleles in Tetranychus urticae, a rapidly evolving pest. We sequence the genomes of 258 T. urticae females collected from China. Combined with global reference genomic data, we examine the evolutionary origin(s) of 18 mutations across 10 target-site genes and analyze the global population genetic structure using genome-wide SNPs. Our findings reveal a striking prevalence of multiple independent origins of resistance mutations, with only two of 18 mutations showing an apparent single origin. Population structure and haplotype analyses point to an important role of gene flow in the spread of resistance alleles. Selection analyses reveal pesticide-driven sweeps affecting genetic diversity. These findings advance our understanding of the rapid adaptation of arthropod herbivores to extreme selective pressure.
抗药性的快速演变给粮食生产带来了巨大压力。然而，抗性等位基因如何产生并在田间种群中传播，在很大程度上仍不为人所知。在此，我们研究了二斑叶螨（一种快速进化的害虫）中抗性等位基因的进化轨迹。我们对从中国采集的258只二斑叶螨雌螨的基因组进行了测序。结合全球参考基因组数据，我们研究了10个靶标位点基因上18个突变的进化起源，并使用全基因组单核苷酸多态性分析了全球种群遗传结构。我们的研究结果显示，抗性突变存在多个独立起源的显著普遍性，18个突变中只有两个显示出明显的单一起源。种群结构和单倍型分析表明基因流在抗性等位基因传播中发挥了重要作用。选择分析揭示了农药驱动的扫荡对遗传多样性的影响。这些发现推进了我们对节肢动物食草动物如何快速适应极端选择压力的理解。
