Zhou Zhe, Zhang Haiqing, Yao Jia-Long, Gao Qiming, Wang Yarong, Liu Zhenzhen, Zhang Yaru, Tian Yi, Yan Zhenli, Zhu Yanmin, Zhang Hengtao
National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan 450009, China.
Zhongyuan Research Center, Chinese Academy of Agricultural Sciences, Xinxiang, Henan 450008, China.
Plant Physiol. 2024 Dec 23;197(1). doi: 10.1093/plphys/kiae518.
Apple replant disease (ARD) is a worldwide problem that threatens the industry. However, the genetic mechanism underlying plant disease resistance against ARD remains unclear. In this study, a negative regulatory microRNA in Malus domestica, mdm-miR397b, and its direct target MdLAC7b (Laccase) was selected for examination based on our previous small RNA and degradome sequencing results. Overexpressing the mdm-miR397b-MdLAC7b module altered the lignin deposition and jasmonic acid contents in apple roots, which also led to increased resistance to Fusarium solani. Additionally, Y1H library screening using mdm-miR397b promoter recombinants identified a transcription factor, MdERF61, that represses mdm-miR397b transcriptional activity by directly binding to 2 GCC-boxes in the mdm-miR397b promoter. In summary, our results suggest that the MdERF61-mdm-miR397b-MdLAC7b module plays a crucial role in apple resistance to F. solani and offers insights for enhancing plant resistance to soil-borne diseases in apples.
苹果再植病（ARD）是一个威胁该产业的全球性问题。然而，植物对ARD抗病性的遗传机制仍不清楚。在本研究中，基于我们之前的小RNA和降解组测序结果，选择了苹果中的一个负调控微小RNA（mdm-miR397b）及其直接靶标MdLAC7b（漆酶）进行研究。过表达mdm-miR397b-MdLAC7b模块改变了苹果根中木质素沉积和茉莉酸含量，这也导致对茄类镰刀菌的抗性增强。此外，使用mdm-miR397b启动子重组体进行的酵母单杂交文库筛选鉴定出一个转录因子MdERF61，它通过直接结合mdm-miR397b启动子中的2个GCC-box来抑制mdm-miR397b的转录活性。总之，我们的结果表明MdERF61-mdm-miR397b-MdLAC7b模块在苹果对茄类镰刀菌的抗性中起关键作用，并为增强苹果对土传病害的抗性提供了见解。
