Ann Biol Clin (Paris). 1985;43(4):476-84.
Pure acetylcholine esterase from Electrophorus Electricus has been covalently coupled to different prostaglandins or thromboxanes. The corresponding conjugates have been tested using microtiter plates with 96 wells coated with a second antibody. The minimal detectable concentrations were 10 pg/ml. We suggest that this procedure which has been extended to non icosanoid molecules may serve as a general technique for enzyme immunoassay.
来自电鳗的纯乙酰胆碱酯酶已与不同的前列腺素或血栓烷共价偶联。相应的偶联物已使用包被有二抗的96孔微量滴定板进行检测。最低可检测浓度为10 pg/ml。我们认为,这种已扩展到非类二十烷酸分子的方法可作为酶免疫测定的通用技术。