Stroeve P, Hoofd L J, Kreuzer F
Ann Biomed Eng. 1985;13(6):551-71. doi: 10.1007/BF02584257.
Steady-state CO2 diffusion in the presence of a CO2 gradient was measured in thin layers of bovine albumin solutions containing different amounts of buffer base, added as NaHCO3, and/or of NaCl. In the same solutions, electrical potentials due to the CO2 diffusion across the layers were measured. Addition of carbonic anhydrase induced a chemical reaction equilibrium to exist for the CO2 reaction system, and led to equilibrium values for facilitated CO2 transport due to a bicarbonate flux and to maximum values for the diffusion potential. The diffusion potentials are generated due to the large differences in the ionic mobilities of albumin and other ionic species such as bicarbonate. The diffusion potential markedly reduces the facilitated CO2 flux. The presence of sodium chloride had no significant effect on the CO2 transport rate. The total mass transfer rates of CO2 in albumin solutions were considerably lower than those found by Stroeve and Ziegler (23) in hemoglobin solutions at identical concentrations of buffer base.
在含有不同量作为 NaHCO₃ 添加的缓冲碱和/或 NaCl 的牛白蛋白溶液薄层中，测量了存在 CO₂ 梯度时的稳态 CO₂ 扩散。在相同溶液中，测量了由于 CO₂ 跨层扩散而产生的电势。添加碳酸酐酶导致 CO₂ 反应系统存在化学反应平衡，并导致由于碳酸氢盐通量促进 CO₂ 运输的平衡值以及扩散电位的最大值。扩散电位是由于白蛋白与其他离子物种（如碳酸氢盐）的离子迁移率差异很大而产生的。扩散电位显著降低了促进的 CO₂ 通量。氯化钠的存在对 CO₂ 传输速率没有显著影响。在相同缓冲碱浓度下，白蛋白溶液中 CO₂ 的总传质速率远低于 Stroeve 和 Ziegler（23）在血红蛋白溶液中发现的速率。
