Rezvan S P, Bekbergenov B M
Antibiot Med Biotekhnol. 1985 Nov;30(11):832-7.
A nutrient medium Unimicon-s for determination of antibiotic sensitivity of microorganisms is described. The medium is based on the raw material not used as a food, i. e. a clarified enzyme lysate of microbial biomass obtained at the All-Union Research Institute for Protein Synthesis. Unimicon-s provided satisfactory growth of various cultures and the sensitivity determination results analogous to those obtained with medium 1 (MPA) or Muller-Hinton medium recommended by the WHO as a standard medium for determination of microbial antibiotic sensitivity. The study on estimation of the fitness of the present criteria for dividing cultures by the sensitivity levels, i. e. into sensitive, moderate sensitive and resistant showed that with the use of an inoculation dose of 2.5 X 10(7) microbial cells per 1 ml the growth inhibition zones of the standard strains on the Unimicon-s medium were within the ranges of the permissible limits for medium 1. The Unimicon-s medium was tested with positive results in the laboratories of the A. N. Bakulev Institute of Cardiovascular Surgery and the 1st N. I. Pirogov City Clinical Hospital.
本文描述了一种用于测定微生物对抗生素敏感性的营养培养基Unimicon-s。该培养基以非食品原料为基础，即全苏蛋白质合成研究所获得的微生物生物质澄清酶解物。Unimicon-s能使各种培养物良好生长，其敏感性测定结果与使用培养基1（MPA）或世界卫生组织推荐的作为测定微生物抗生素敏感性标准培养基的Muller-Hinton培养基所得结果相似。关于评估按敏感性水平（即分为敏感、中度敏感和耐药）划分培养物的现行标准适用性的研究表明，每1 ml接种剂量为2.5×10⁷个微生物细胞时，标准菌株在Unimicon-s培养基上的生长抑制圈在培养基1的允许范围内。Unimicon-s培养基在A. N. 巴库列夫心血管外科研究所和第一尼·伊·皮罗戈夫市临床医院的实验室进行了测试，结果为阳性。
