Comparison of ribosomes from gram-positive and gram-negative bacteria with respect to the presence of protein S1.

Ribosomes from Gram-positive and Gram-negative bacteria have been analysed for the presence of ribosomal protein S1 by three methods, sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoreaction with E. coli anti-S1 and chromatography on poly (U)-Sepharose. We observed that protein S1 is predominantly present in Gram-negative bacteria in comparison with Gram-positive bacteria. Exceptions are noted in both species.