采用双位点酶免疫分析法对包括恶性肿瘤在内的各种胃肠道疾病患者尿液中的人表皮生长因子（尿抑胃素）进行检测。
Assessment by a two-site enzyme immunoassay of human epidermal growth factor (urogastrone) in the urine of patients with various gastrointestinal diseases including malignant tumors.
作者信息
Kurobe M, Aono M, Moriga M, Furukawa S, Hayashi K
出版信息
Biochem Int. 1985 Dec;11(6):817-23.
By using our two-site enzyme immunoassay (EIA) system, the levels of human epidermal growth factor (hEGF) in the urine of patients with various gastrointestinal diseases including malignant tumors were measured. Urinary excretion of hEGF in patients having undergone gastric resection, expressed as a function of creatinine, was found to be somewhat decreased. While the levels of hEGF in patients with gastric cancer were significantly increased. Then, the molecular features of hEGF in the urine of patients with gastric cancer were examined by gel filtration. The elution profile demonstrated that high molecular weight components, which immunologically cross-reacted with hEGF, were considerably increased. On the other hand, the level of normal EGF with a molecular weight of 6500 was decreased to some extent. These results suggest that processing of the EGF precursor into an active EGF molecule is partially suppressed in patients with gastric cancer.
通过使用我们的双位点酶免疫分析（EIA）系统，对包括恶性肿瘤在内的各种胃肠道疾病患者尿液中的人表皮生长因子（hEGF）水平进行了测量。胃切除患者尿液中hEGF的排泄量以肌酐为函数表示，发现有所下降。而胃癌患者的hEGF水平显著升高。然后，通过凝胶过滤检查了胃癌患者尿液中hEGF的分子特征。洗脱图谱表明，与hEGF发生免疫交叉反应的高分子量成分显著增加。另一方面，分子量为6500的正常EGF水平在一定程度上有所下降。这些结果表明，胃癌患者中EGF前体加工成活性EGF分子的过程受到部分抑制。
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