磷酸钙陶瓷的物理化学性质对间充质干细胞的生物相容性和成骨分化的影响。
The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells.
机构信息
Department of Internal Medicine, Medical Oncology & Pulmonology, VIII (AG Zender), University Clinic Tübingen, Otfried-Müller-Str. 14, Tuebingen, 72076, Germany.
Department of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Akad. Zabolotnogo str, Kyiv, 03680, Ukraine.
出版信息
BMC Res Notes. 2024 Oct 7;17(1):295. doi: 10.1186/s13104-024-06937-y.
OBJECTIVE
In this study we have focused on biocompatibility and osteoinductive capacity analysis of self-manufactured single-phase (HAP) and two-phase (HAP and β-ТСР) bioactive ceramics with various chemical modifications (Fig. 1).
RESULTS
We demonstrate a reduction in solubility for all analyzed composite after the treatment with HO and HO, accompanied by an enhancement in adsorption activity. This modification also resulted in an increase in micro- and macroporosity, along with a rise in the open porosity. Adipose-derived mesenchymal stromal cells demonstrated excellent cell adhesion and survival when cultured with these ceramics. Calcium phosphate ceramics (H-500, HT-500, and HT-1 series) stimulated alkaline phosphatase expression, promoted calcium deposition, and enhanced osteopontin expression in ADSCs, independently inducing osteogenesis without additional osteogenic stimuli. These findings underscore the promising potential of HAP-based bioceramics for bone regeneration/reconstruction.
目的
在本研究中，我们专注于分析自制单相（HAP）和双相（HAP 和 β-ТСР）生物活性陶瓷的生物相容性和骨诱导能力，并对其进行了各种化学修饰（图 1）。
结果
我们证明，在用 HO 和 HO 处理后，所有分析的复合材料的溶解度都降低了，同时吸附活性增强。这种修饰还导致微多孔和大孔的增加，以及开放孔隙率的增加。脂肪来源的间充质基质细胞在与这些陶瓷培养时表现出良好的细胞黏附和存活。钙磷酸盐陶瓷（H-500、HT-500 和 HT-1 系列）可刺激碱性磷酸酶的表达，促进 ADSC 中钙的沉积，并增强骨桥蛋白的表达，独立诱导成骨而无需额外的成骨刺激。这些发现突出了基于 HAP 的生物陶瓷在骨再生/重建方面的巨大潜力。
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