Apaydın Emre, Yaşar Buse, Şimşek Gülçin, Kaygın Pınar, Sarıaltın Sezen Yılmaz, Dirican Onur, Çetin Hazal Eylem, Husseini Abbas Ali, Oğuztüzün Serpil
Department of Otorhinolaryngology, Health Sciences University, Atatürk Sanatorium Training and Research Hospital, Ankara, Türkiye.
Departmet of Pathology, University of Health Sciences, Gulhane Training and Research Hospital, Ankara, Türkiye.
Indian J Otolaryngol Head Neck Surg. 2024 Oct;76(5):4516-4522. doi: 10.1007/s12070-024-04900-8. Epub 2024 Jul 18.
Adenoid hypertrophy is a prevalent pediatric condition, often necessitating surgical intervention. Intranasal steroid administration shows promise as a conservative treatment, particularly by inducing apoptosis in adenoidal cells, leading to a reduction in adenoid size and inflammation. This study aims to characterize the expression profile of caspase-3 as an apoptotic inducer protein in inflammatory and epithelial adenoid tissues and explore its association with steroid administration.
We performed immunohistochemical staining for caspase-3 proteins in adenoid tissues obtained from 51 pediatric patients aged between 2.5 and 12 years (mean age: 6.09 ± 2.1 years) who underwent adenoid surgery. A retrospective analysis of clinical data was conducted, categorizing participants into steroid treatment receivers ( = 25) and non-receivers ( = 26). Subsequently, the lymphoid inflammatory tissue and epithelial tissue from the adenoid were compared in terms of caspase-3 protein expression, and associated clinical variables were assessed.
Immunohistochemical analysis revealed significant caspase-3 expression in inflammatory tissues. The expression levels were scored, and no significant correlation was observed between inflammation and epithelium based on caspase-3 expression (correlation coefficient = 0.143; > 0.05). Furthermore, demographic and clinical characteristics did not show a statistically significant difference in caspase-3 expression levels.
Caspase-3 expression was significant in inflammatory adenoid tissue, but it showed no association with nasal steroid administration.
腺样体肥大是一种常见的儿科疾病，常需手术干预。鼻内给予类固醇作为一种保守治疗方法显示出前景，特别是通过诱导腺样体细胞凋亡，导致腺样体大小减小和炎症减轻。本研究旨在表征半胱天冬酶 - 3作为凋亡诱导蛋白在炎性和上皮性腺样体组织中的表达谱，并探讨其与类固醇给药的关联。
我们对51例年龄在2.5至12岁（平均年龄：6.09±2.1岁）接受腺样体手术的儿科患者的腺样体组织进行了半胱天冬酶 - 3蛋白的免疫组织化学染色。对临床资料进行回顾性分析，将参与者分为接受类固醇治疗者（n = 25）和未接受者（n = 26）。随后，比较腺样体的淋巴样炎性组织和上皮组织中半胱天冬酶 - 3蛋白表达情况，并评估相关临床变量。
免疫组织化学分析显示炎性组织中有显著的半胱天冬酶 - 3表达。对表达水平进行评分，基于半胱天冬酶 - 3表达，炎症与上皮之间未观察到显著相关性（相关系数 = 0.143；P>0.05）。此外，人口统计学和临床特征在半胱天冬酶 - 3表达水平上未显示出统计学显著差异。
半胱天冬酶 - 3在炎性腺样体组织中表达显著，但与鼻用类固醇给药无关。
