修饰氨基酸调控转录活性的单分子检测

Komoto Yuki, Ohshiro Takahito, Notsu Yuno, Taniguchi Masateru

SANKEN, Osaka University 8-1, Mihogaoka Ibaraki Osaka 567-0047 Japan

Artificial Intelligence Research Center, Osaka University 8-1 Mihogaoka, Ibaraki Osaka 567-0047 Japan.

RSC Adv. 2024 Oct 7;14(43):31740-31744. doi: 10.1039/d4ra05488a. eCollection 2024 Oct 1.

Acetylation of lysine, a component of histones, regulates transcriptional activity. Simple detection methods for acetyl lysine are essential for early diagnosis of diseases and understanding of the physiological effects. We have detected and recognized acetyl lysine at the single-molecule level by combining MCBJ measurement and machine learning.

赖氨酸（组蛋白的一个组成部分）的乙酰化作用可调节转录活性。乙酰赖氨酸的简易检测方法对于疾病的早期诊断和生理效应的理解至关重要。我们通过结合微量恒定电流脉冲法（MCBJ）测量和机器学习，在单分子水平上检测并识别了乙酰赖氨酸。