Hathnagoda Randi, Gunathilake Pinidi, Buddhinee Thilini, Welgama Pabasara, Gunarathna Hasini, Perera Harshani, Ranasinghe Koshila
Department of Biomedical Sciences, Faculty of Health Sciences, CINEC Campus, Malabe, Sri Lanka.
Department of Zoology and Environmental Management, Faculty of Science, University of Kelaniya, Dalugama, Kelaniya GQ 11600, Sri Lanka.
J Trop Med. 2024 Jul 25;2024:1832200. doi: 10.1155/2024/1832200. eCollection 2024.
Mosquitoes, notorious for their deadly impact as disease vectors, also hold economic value owing to their roles in disease transmission. The present study focuses on the importance of understanding mosquito gut microbiota for implementing innovative vector control strategies, thereby mitigating disease transmission. The study was conducted in the Gampaha Medical Office of Health (MOH) area of Sri Lanka with the focus of elucidating the microbial diversity within the midgut of , a crucial step to support ongoing paratransgenesis efforts. Sampling was performed by utilizing standard mosquito sampling techniques and their midgut homogenates were plated on Plate Count Agar to isolate bacteria, which were then identified through biochemical tests. Subsequently, the most abundant bacterial families were subjected to DNA extraction, PCR amplification, and gene sequencing for species identification. The study revealed the presence of four bacterial families (Staphylococcaceae, Streptococcaceae, Neisseriaceae, and Moraxellaceae) in adult mosquitoes, while larvae harbored an additional family, Micrococcaceae. Interestingly, the relative distribution of midgut bacteria varied significantly among field-caught larval and adult strains from different study areas (chi-square = 1.673; < 0.05), indicating similar bacterial flora across mosquito life stages and geographical locations. Of particular interest is the identification of , a bacterium with potential for paratransgenesis applications. Given the high mosquito density in the study area, leveraging paratransgenesis for control is recommended. Furthermore, insights into gut microbes could inform the integration of gut microflora from modified strains into existing Sterile Insect Technique (SIT) and Incompatible Insect Technique (IIT) approaches in Sri Lanka.
蚊子作为疾病传播媒介，其致命影响臭名昭著，但因其在疾病传播中的作用也具有经济价值。本研究着重于了解蚊子肠道微生物群对于实施创新病媒控制策略的重要性，从而减轻疾病传播。该研究在斯里兰卡甘帕哈卫生医疗办公室（MOH）地区开展，重点是阐明[具体蚊子种类]中肠内的微生物多样性，这是支持正在进行的共生转基因研究工作的关键一步。采用标准的蚊子采样技术进行采样，将其肠道匀浆接种在平板计数琼脂上以分离细菌，然后通过生化试验进行鉴定。随后，对最丰富的细菌科进行DNA提取、PCR扩增和基因测序以进行物种鉴定。研究发现成年蚊子中存在四个细菌科（葡萄球菌科、链球菌科、奈瑟菌科和莫拉克斯菌科），而幼虫中还含有另一个科，微球菌科。有趣的是，不同研究区域野外捕获的幼虫和成年菌株中肠细菌的相对分布差异显著（卡方检验=1.673；P<0.05），表明蚊子不同生命阶段和地理位置的细菌菌群相似。特别值得关注的是[具体细菌名称]的鉴定，这是一种具有共生转基因应用潜力的细菌。鉴于研究区域蚊子密度高，建议利用共生转基因进行[蚊子种类]控制。此外，对肠道微生物的深入了解可为将改良菌株的肠道微生物群整合到斯里兰卡现有的昆虫不育技术（SIT）和昆虫不相容技术（IIT）方法中提供参考。
