丝状真菌乳白抑制tRNA基因的克隆揭示了两个具有意外结构差异的tRNASerUGA基因。
Cloning of opal suppressor tRNA genes of a filamentous fungus reveals two tRNASerUGA genes with unexpected structural differences.
作者信息
Debuchy R, Brygoo Y
出版信息
EMBO J. 1985 Dec 16;4(13A):3553-6. doi: 10.1002/j.1460-2075.1985.tb04116.x.
Abstract
The informational suppressors su4-1 and su8-1 of Podospora anserina were isolated by transformation of Schizosaccharomyces pombe UGA mutants. The DNA sequence revealed that they were opal (UGA) suppressor tRNAs. Wild-type alleles were also isolated by hybridization. The DNA sequence showed that they both encode species of tRNASerUGA. The gene SU8 has an 18-bp intervening sequence and its primary sequence is very different from that of SU4.
摘要
通过对粟酒裂殖酵母UGA突变体进行转化，分离得到了嗜热栖热放线菌的信息抑制因子su4-1和su8-1。DNA序列显示它们是乳白（UGA）抑制性tRNA。通过杂交也分离得到了野生型等位基因。DNA序列表明它们都编码tRNASerUGA物种。基因SU8有一个18bp的间隔序列，其一级序列与SU4的序列有很大不同。
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