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六个粟酒裂殖酵母tRNA基因,包括一个带有间隔序列的tRNALys基因,该间隔序列不能与反密码子进行碱基配对。

Six Schizosaccharomyces pombe tRNA genes including a gene for a tRNALys with an intervening sequence which cannot base-pair with the anticodon.

作者信息

Gamulin V, Mao J, Appel B, Sumner-Smith M, Yamao F, Söll D

出版信息

Nucleic Acids Res. 1983 Dec 20;11(24):8537-46. doi: 10.1093/nar/11.24.8537.

Abstract

We report the sequences of six S. pombe tRNA genes including two genes for tRNAArg, and one gene each for tRNAGlu, tRNAHis, tRNALys and tRNAPhe. All tRNA genes are found independently in the genome and represent individual transcription units. The gene for tRNALys has an 8 bp long intervening sequence which cannot base-pair with the tRNA anticodon. In vitro transcription studies indicate that all genes are faithfully transcribed in a yeast extract. Sequence comparison of the 5' flanking regions of the tRNA genes did not show significant homologies; however, they are very rich in AT base pairs.

摘要

我们报道了六个粟酒裂殖酵母tRNA基因的序列,其中包括两个tRNAArg基因,以及tRNAGlu、tRNAHis、tRNALys和tRNAPhe各一个基因。所有tRNA基因均独立存在于基因组中,代表单个转录单元。tRNALys基因有一段8 bp长的间隔序列,该序列不能与tRNA反密码子形成碱基对。体外转录研究表明,所有基因在酵母提取物中都能被准确转录。tRNA基因5'侧翼区域的序列比较未显示出显著的同源性;然而,它们富含AT碱基对。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f94/326605/17faef7bc18c/nar00369-0019-a.jpg

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