Longping Branch, College of Biology, Hunan University, Changsha 410125, China.
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
J Agric Food Chem. 2024 Oct 30;72(43):23758-23765. doi: 10.1021/acs.jafc.4c07765. Epub 2024 Oct 8.
Intensive application of glyphosate has resulted in resistance evolution in many weed populations, including . This study characterized glyphosate resistance and investigated the underlying mechanisms in a glyphosate-resistant population (R-JX) of from China. The R-JX population was 8.5 times resistant to glyphosate relative to the glyphosate-susceptible population (SA). Point mutations were not observed in the target gene 5-enolypyruvyl-shikimate-3-phosphate synthase gene (). However, the expression level and copy number of were 8.8 times and 15.2 times, respectively, greater in R-JX than that in the SA population. Pre-application of the P450 inhibitor lowered the resistance level to glyphosate from 8.5 times to 3.6 times in the R-JX population. RNA-Seq and RT-qPCR revealed that the gene was consistently upregulated in R-JX and five other glyphosate-resistant populations. Rice calli and seedlings overexpressing showed glyphosate resistance. In conclusion, overexpression of the target plus collectively contributes to glyphosate resistance in these populations.
草甘膦的大量使用导致包括 在内的许多杂草种群产生了抗药性。本研究对来自中国的抗草甘膦 种群 (R-JX) 进行了草甘膦抗性特征描述和抗性机制研究。与草甘膦敏感种群 (SA) 相比，R-JX 种群对草甘膦的抗性高出 8.5 倍。靶基因 5-烯醇丙酮酰莽草酸-3-磷酸合酶基因 () 中未观察到点突变。然而，R-JX 中的表达水平和拷贝数分别比 SA 种群高 8.8 倍和 15.2 倍。在 R-JX 种群中，预先应用 P450 抑制剂可将草甘膦的抗性水平从 8.5 倍降低至 3.6 倍。RNA-Seq 和 RT-qPCR 显示，在 R-JX 和其他五个抗草甘膦种群中， 基因始终被上调。过表达 基因的水稻愈伤组织和幼苗表现出抗草甘膦性。总之，这些 种群对草甘膦的抗性是由靶标 基因的过表达加上 基因的共同作用所致。
