Department of Obstetrics and Gynaecology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Department of Urology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Microbiol Spectr. 2024 Nov 5;12(11):e0030324. doi: 10.1128/spectrum.00303-24. Epub 2024 Oct 8.
is a major skin commensal that may act as an opportunistic pathogen. It is difficult to interpret findings of in tissue cultures obtained during arthroplasty revision surgery, since they may represent true infection or contamination. This study investigated whether obtained from prosthetic joint infections (PJIs) were related and shared common genomic traits that might correlate with clinical courses and patient outcomes. isolates from revision surgery of patients with PJIs of the hip, shoulder, and knee were characterized using molecular methods to determine the sequence type (ST) and the presence of possible virulence determinants (Christie-Atkins-Munch-Peterson factors, dermatan sulfate-binding adhesion 1, hyaluronidase lyase, and linear plasmid). A standardized review of the patients' medical charts was performed. The study included 37 patients with culture-positive tissue samples where multiple isolates of belonged to the same ST. Most of the isolates belonged to phylotype IA. Phylogenetic analysis of virulence determinants revealed no shared pattern among PJI isolates. Seven patients had a polymicrobial infection. Exchange revision was performed in 70% of the patients, and >50% of all patients received antibiotic treatment for ≥3 months. Failure was noted in seven patients. No specific ST or any identifiable unique feature among virulence determinants were found among isolated from PJIs of hips and shoulders. The majority of patients had low inflammatory markers and were treated successfully, even polymicrobial infections. However, failure was more common among shoulder infections compared with hip infections.
Prosthetic joint infection (PJI) is a rare complication after arthroplasty surgery. The infection seldom resolves without a combination of both surgical and antibiotic treatment and can cause significant suffering among affected patients. is a common skin bacterium that is most often found in shoulder PJIs but can also infect other prostheses. In this study, we conducted a review of patients with previously verified PJIs involving in hip or shoulder prostheses, along with a genomic analysis of the bacteria causing the infections. The majority of patients had successful outcomes. We did not identify any specific phylogenetic lineage or specific molecular signature of virulence factors among these PJI-associated isolates that seemed to be associated with increased potential to cause infection among this species. This indicates that isolated from PJIs originates from the patients' own skin microbiome and is inoculated during the arthroplasty surgery.
是一种主要的皮肤共生菌，它可能是一种机会性病原体。在关节置换翻修手术中获得的组织培养物中，很难解释 的发现，因为它们可能代表真正的感染或污染。本研究调查了从假体关节感染（PJI）中获得的 是否与共同的基因组特征相关，这些特征可能与临床过程和患者结局相关。使用分子方法对来自髋关节、肩关节和膝关节 PJI 翻修手术患者的 分离株进行特征描述，以确定序列型（ST）和可能的毒力决定因素（Christie-Atkins-Munch-Peterson 因子、硫酸皮肤素结合黏附素 1、透明质酸酶裂解酶和线性质粒）的存在。对患者病历进行了标准化回顾。该研究纳入了 37 名 培养阳性组织样本的患者，其中多个 分离株属于同一 ST。大多数分离株属于表型 IA。毒力决定因素的系统发育分析显示，PJI 分离株之间没有共同的模式。7 名患者存在混合感染。70%的患者进行了翻修交换，超过 50%的患者接受了≥3 个月的抗生素治疗。7 名患者出现治疗失败。在髋关节和肩关节 PJI 中分离的 中没有发现特定的 ST 或任何可识别的毒力决定因素的独特特征。大多数患者的炎症标志物较低，治疗效果良好，即使是混合感染。然而，与髋关节感染相比，肩部感染的失败更为常见。
假体关节感染（PJI）是关节置换手术后罕见的并发症。如果不结合手术和抗生素治疗，感染很少能自行消退，这会给受影响的患者带来严重的痛苦。 是一种常见的皮肤细菌，最常发现于肩部 PJI，但也可感染其他假体。在这项研究中，我们对先前确诊的髋关节或肩部假体 PJI 患者进行了回顾，并对引起感染的细菌进行了基因组分析。大多数患者的治疗效果良好。我们没有发现与该物种感染潜力增加相关的特定系统发育谱系或特定毒力因子分子特征。这表明，从 PJI 中分离出的 来自患者自身的皮肤微生物组，并在关节置换手术中接种。
