Aubin Guillaume Ghislain, Lavigne Jean-Philippe, Foucher Yohan, Dellière Sarah, Lepelletier Didier, Gouin François, Corvec Stéphane
EA3826, Laboratory of Clinical and Experimental Therapeutics of Infections, IRS 2, Nantes University, Nantes, France; Bacteriology and Hygiene Unit, CHU Nantes, France.
INSERM, U1047, University of Montpellier, Nîmes, France; Department of Microbiology, Caremeau University Hospital, Nîmes, France.
Anaerobe. 2017 Oct;47:73-78. doi: 10.1016/j.anaerobe.2017.04.009. Epub 2017 Apr 25.
The recognition of the pathogenicity of Cutibacterium acnes in implant-associated infection is not always obvious. In this paper, we aimed to distinguish pathogenic and non-pathogenic C. acnes isolates. To reach this goal, we investigated the clonal complex (CC) of a large collection of C. acnes clinical isolates through Multi-Locus Sequence Typing (MLST), we established a Caenorhabditis elegans model to assess C. acnes virulence and we investigated the presence of virulence factors in our collection. Ours results showed that CC36 and CC53 C. acnes isolates were more frequently observed in prosthetic joint infections (PJI) than CC18 and CC28 C. acnes isolates (p = 0.021). The C. elegans model developed here showed two distinct virulence groups of C. acnes (p < 0.05). These groups were not correlated to CC or clinical origin. Whole genome sequencing allowed us to identify a putative gene linked to low virulent strains. In conclusion, MLST remains a good method to screen pathogenic C. acnes isolates according to their clinical context but mechanisms of C. acnes virulence need to be assess thought transcriptomic analysis to investigate regulatory process.
痤疮丙酸杆菌在植入物相关感染中的致病性并非总是显而易见。在本文中，我们旨在区分致病性和非致病性痤疮丙酸杆菌分离株。为实现这一目标，我们通过多位点序列分型（MLST）研究了大量痤疮丙酸杆菌临床分离株的克隆复合体（CC），建立了秀丽隐杆线虫模型来评估痤疮丙酸杆菌的毒力，并研究了我们所收集菌株中毒力因子的存在情况。我们的结果表明，与CC18和CC28痤疮丙酸杆菌分离株相比，CC36和CC53痤疮丙酸杆菌分离株在人工关节感染（PJI）中更常被观察到（p = 0.021）。在此开发的秀丽隐杆线虫模型显示出痤疮丙酸杆菌的两个不同毒力组（p < 0.05）。这些组与克隆复合体或临床来源无关。全基因组测序使我们能够鉴定出一个与低毒力菌株相关的假定基因。总之，MLST仍然是根据临床背景筛选致病性痤疮丙酸杆菌分离株的好方法，但痤疮丙酸杆菌毒力机制需要通过转录组分析来评估，以研究调控过程。
