Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, Zhengzhou University, School of Pharmaceutical Sciences, Zhengzhou 450001, China.
J Chromatogr A. 2024 Nov 8;1736:465420. doi: 10.1016/j.chroma.2024.465420. Epub 2024 Oct 5.
This study delineates the development of a novel automated pipette-tip solid-phase extraction (SPE) methodology, employing kapok fiber as a naturally efficient and cost-effective adsorbent for the selective extraction of eleven tyrosine kinase inhibitors (TKIs) from plasma. The uniqueness of this method lies in its assembly, where kapok fibers are ingeniously wrapped around a stainless-steel spring within the pipette tip, ensuring an obstruction-free central space for effortless solution aspiration and dispensation. This design significantly minimizes backpressure, enhancing operational efficiency and ensuring compatibility with pipettors, including the implementation of an electric pipettor to streamline the sample preparation process and facilitate automation. The method's analytical performance, rigorously validated through liquid chromatography-tandem mass spectrometry, exhibits outstanding linearity in ranges of 0.1/0.5-200 ng mL (R² > 0.993), commendable accuracy (86.3%-114.8%), and consistent precision (3.4-11.3%), alongside remarkably low detection limits that span from 0.024 to 0.130 ng mL. The assembly of kapok fiber within the pipette tip, in this unique configuration, results in a practical, cost-effective, eco-friendly, and automated pipette-tip SPE method. This innovation signifies a significant advancement in bioanalytical methodologies, offering an efficient and sustainable approach for extracting analytes from complex biological samples. This process notably enhances both the sensitivity and selectivity of subsequent instrumental analyses.
本研究开发了一种新颖的自动化移液管尖端固相萃取（SPE）方法，使用木棉纤维作为一种天然高效且经济实惠的吸附剂，从血浆中选择性提取 11 种酪氨酸激酶抑制剂（TKIs）。该方法的独特之处在于其组装，巧妙地将木棉纤维包裹在移液管尖端内的不锈钢弹簧周围，确保中央空间无障碍，便于轻松吸取和解吸溶液。这种设计可显著减小反压，提高操作效率，并确保与移液管兼容，包括使用电动移液管来简化样品制备过程并促进自动化。该方法的分析性能通过液相色谱-串联质谱法进行了严格验证，在 0.1/0.5-200ng/mL 范围内表现出出色的线性（R²>0.993）、令人满意的准确度（86.3%-114.8%）和一致的精密度（3.4-11.3%），并且检测限非常低，范围为 0.024-0.130ng/mL。在这种独特的配置中，将木棉纤维组装在移液管尖端内，形成了一种实用、经济实惠、环保且自动化的移液管尖端 SPE 方法。这项创新标志着生物分析方法的重大进展，为从复杂生物样品中提取分析物提供了一种高效且可持续的方法。该过程显著提高了后续仪器分析的灵敏度和选择性。
