Department of Human Anatomy, School of Basic Medicine, Binzhou Medical University, Yantai, 264003, China.
Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China.
Anal Chim Acta. 2024 Nov 15;1329:343240. doi: 10.1016/j.aca.2024.343240. Epub 2024 Sep 11.
The detection of tyrosine kinase inhibitors (TKIs) in biological fluids is essential due to their critical role in cancer therapy and the variability in individual drug metabolism, which necessitates precise dosing. Traditional methods for analyzing TKIs in biological fluids, such as blood plasma, typically involve complex sample preparation techniques that can be resource-intensive, environmentally burdensome, and not sufficiently sensitive for low-concentration analytes. There is a pressing need for more efficient, economical, and environmentally friendly methods that can enhance sensitivity and throughput without compromising accuracy.
This study explores the use of melt-blown polypropylene nonwoven (MBPP), commonly found in face masks, as a novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE). MBPP demonstrated excellent hydrophobicity and significant mesoporous adsorption capacity. An extraction device was fashioned by inserting a segment of MBPP (15 mg) into a 200 μL disposable plastic pipette tip, which was then attached to a 2.5 mL disposable plastic syringe. The MBPP's fabric form removes the need for a frit, allowing the extraction process to be completed in just 3 min through simple plunger manipulation. The method achieved extraction recoveries ranging from 60.5 % to nearly 100 %. Subsequent method validation using liquid chromatography-tandem mass spectrometry (LC-MS/MS) showed satisfactory linearity (coefficient of determination R > 0.993), accuracy (relative recoveries: 86.3%-114.8 %), and precision (relative standard deviation: 3.4%-11.3 %), with detection limits between 0.022 and 0.135 ng mL.
The introduction of MBPP for PT-μSPE represents a significant advancement in the bioanalytical detection of TKIs, offering a highly efficient, cost-effective, and environmentally sustainable method. It compares favorably with existing techniques, offering advantages in terms of cost, environmental impact, and ease of use. This approach has the potential to be widely adopted for routine monitoring of TKIs in clinical settings.
由于酪氨酸激酶抑制剂 (TKI) 在癌症治疗中的关键作用以及个体药物代谢的变异性，需要精确的剂量，因此在生物体液中检测 TKI 至关重要。传统的分析生物体液中 TKI 的方法，如血浆，通常涉及复杂的样品制备技术，这些技术可能需要大量资源，对环境造成负担，并且对低浓度分析物的灵敏度不够。因此，迫切需要更高效、经济和环保的方法，在不影响准确性的情况下提高灵敏度和通量。
本研究探讨了将熔喷聚丙烯非织造布（MBPP）（通常用于口罩）用作新型微量固相萃取（μSPE）的萃取剂。MBPP 表现出极好的疏水性和显著的中孔吸附能力。通过将 15mg 的 MBPP 插入 200μL 一次性塑料移液管吸头中，制成萃取装置，然后将其连接到 2.5mL 一次性塑料注射器上。MBPP 的织物形式不需要使用滤片，只需通过简单的柱塞操作，3 分钟即可完成萃取过程。该方法的提取回收率在 60.5%到近 100%之间。随后使用液相色谱-串联质谱法（LC-MS/MS）进行方法验证，结果表明具有令人满意的线性（决定系数 R>0.993）、准确度（相对回收率：86.3%-114.8%）和精密度（相对标准偏差：3.4%-11.3%），检测限在 0.022-0.135ng/mL 之间。
将 MBPP 引入 PT-μSPE 代表了 TKI 生物分析检测方面的重大进展，提供了一种高效、经济和环保的方法。它与现有技术相比具有成本、环境影响和易用性方面的优势。这种方法有可能在临床环境中广泛用于常规 TKI 监测。
