School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Departament of Oral and Maxillofacial Surgery, HCPA, Porto Alegre, Rio Grande do Sul, Brazil.
J Oral Rehabil. 2024 Nov;51(11):2289-2296. doi: 10.1111/joor.13814. Epub 2024 Aug 2.
Chondroitin and glucosamine sulphates (CGS) are considered structure-modifying drugs and have been studied in the prevention, delay or reversal of structural morphological changes in joints caused by osteoarthritis.
The aim of the present study was to investigate the action of CGS on the progression of chemically induced osteoarthritis in the temporomandibular joint (TMJ) of rabbits by evaluating the serum levels of tumour necrosis factor (TNF-α) and collagen in the articular discs.
A sample of 36 male rabbits was divided into three groups: control (CG), osteoarthritis (OG) and treatment (TG). The disease was induced by intra-articular injection of sodium monoiodoacetate (10 mg/mL) in the OG and TG groups bilaterally. After 10 days, the TG animals received subcutaneous injection of chondroitin sulphates and glucosamine (7.5 mg/kg) and the OG and CG received saline solution (50 μL). Euthanasia times were subdivided into 40 and 100 days. Collagen quantification was performed by biochemical and histological analysis and for the quantification of serum levels of TNF-α, an enzyme immunoassay was used.
The TG showed an increase in the collagen area of the articular disc when compared to the CG and the OG. The increase collagen concentration in the discs did not show a statistically significant difference between the groups. Post-treatment TNF-α levels were significantly lower in TG compared to OG.
The results indicate that CGS treatment delayed the degeneration of the collagen in the TMJ articular disc and reduced serum TNF-α levels, indicating a preventive effect on OA progression.
硫酸软骨素和氨基葡萄糖（CGS）被认为是结构修饰药物，已被研究用于预防、延缓或逆转骨关节炎引起的关节结构形态变化。
本研究旨在通过评估关节盘血清肿瘤坏死因子（TNF-α）和胶原水平，研究 CGS 对兔颞下颌关节（TMJ）化学诱导性骨关节炎进展的作用。
将 36 只雄性兔子分为三组：对照组（CG）、骨关节炎组（OG）和治疗组（TG）。OG 和 TG 组双侧关节内注射单碘酸钠（10mg/mL）诱导疾病。10 天后，TG 组动物接受硫酸软骨素和氨基葡萄糖（7.5mg/kg）皮下注射，OG 和 CG 组接受生理盐水（50μL）。安乐死时间分为 40 天和 100 天。通过生化和组织学分析进行胶原定量，通过酶联免疫吸附试验检测血清 TNF-α 水平。
与 CG 和 OG 相比，TG 组关节盘胶原面积增加。各组间关节盘胶原浓度增加无统计学差异。与 OG 相比，TG 组治疗后 TNF-α 水平显著降低。
结果表明，CGS 治疗可延缓 TMJ 关节盘胶原退变，降低血清 TNF-α 水平，对 OA 进展具有预防作用。
