Department of Pharmacology, School of Pharmaceutical Sciences, Siksha 'O' Anusandhan Deemed to be University, Kalinga Nagar, 751003, Bhubaneswar, Odisha, India.
Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Cell Mol Biol (Noisy-le-grand). 2024 Oct 8;70(9):156-169. doi: 10.14715/cmb/2024.70.9.22.
Fresh stem bark decoction of Litsea monopetala has been practiced for the treatment of jaundice and other liver disorders by the tribal communities of Thakht-e-Sulaiman hills from West Pakistan. As per the folkloric claim, this study aims to identify the phytoconstituents and evaluate the hepatoprotective action of stem bark methanol extract of L. monopetala (LMME). The in-vitro hepatoprotective effect of L. monopetala was performed by H2O2-induced toxicity in the HepG2 cell line and in-vivo by cclt;sub>4-induced hepatotoxicity in Wistar albino rats taking Silymarin as standard drug. Phytoconstituents were identified using LC-QTOF-MS analysis followed by in-silico docking and validation. Molecular docking interactions between identified compounds of L. monopetala and two target proteins, namely 1VJY and 5HYK were presented. In this study, treatment with LMME at 100 µg/mL showed 67.73 % cell viability as compared to H2O2 (100 µM) treated alone i.e., 18.55 % in the HepG2 cell line. In-vivo treatment of LMME reversed the altered serum biochemical parameters and reduced the inflammatory response similar to that of the Silymarin-treated group supported by histopathological investigation. This research reveals that L. monopetala is a rich source of flavonoids and phenols which supports its hepatoprotective effects and is proposed for its usage as a promising hepatoprotective agent after controlled trials.
巴基斯坦西部 Thakht-e-Sulaiman 山区的部落社区一直使用鲜莲叶茎皮煎剂治疗黄疸和其他肝脏疾病。根据民间传说，本研究旨在鉴定莲叶茎甲醇提取物（LMME）的植物成分并评估其对肝脏的保护作用。通过 H2O2 诱导 HepG2 细胞系中的毒性和 CCL4 诱导 Wistar 白化大鼠的肝毒性，在体外和体内研究莲叶的肝保护作用，将水飞蓟素作为标准药物。使用 LC-QTOF-MS 分析鉴定植物成分，然后进行计算机对接和验证。提出了莲叶中鉴定出的化合物与两种靶蛋白 1VJY 和 5HYK 之间的分子对接相互作用。在这项研究中，与单独用 H2O2（100μM）处理相比，LMME 在 100μg/mL 时的细胞存活率为 67.73%，即 HepG2 细胞系中的 18.55%。LMME 的体内治疗可逆转血清生化参数的改变，并减轻炎症反应，与水飞蓟素治疗组相似，这一结果得到了组织病理学研究的支持。这项研究表明，莲叶是黄酮类化合物和酚类化合物的丰富来源，这支持了其对肝脏的保护作用，并建议在经过对照试验后将其用作有前途的肝脏保护剂。
