Bahrami Mahrokh, Bostanghadiri Narjess, Goudarzi Mehdi, Khodaei Niloufar, Hashemi Ali
Department of Microbiology School of Medicine Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Microbiology School of Medicine Iran University of Medical Sciences, Tehran, Iran.
Int J Microbiol. 2024 Oct 1;2024:8224242. doi: 10.1155/2024/8224242. eCollection 2024.
causes challenging infections in immunocompromised patients, exhibiting increasing resistance to multiple antimicrobials and possessing various virulence genes, including emerging resistance to trimethoprim-sulfamethoxazole. A total of 80 clinical isolates of were collected from multiple hospitals in Tehran, Iran. This study conducted an analysis of antibiotic susceptibility by disc diffusion method and -test assay, resistance and virulence gene frequencies were examined by PCR-sequencing, and multilocus sequencing typing (MLST) was performed for strain typing. Across the tested isolates, we observed notably high resistance rates for imipenem 80 (100%), meropenem 78(97.5%), and ceftazidime 72 (90%), while trimethoprim-sulfamethoxazole (SXT) showed a lower resistance rate of 2 (2.5%). Minocycline and levofloxacin demonstrated the highest susceptibility rates, with 70 (87.5%) and 80 (100%), respectively. The prevalence of antibiotic resistance genes bla , and bla was 71 (88.75%) and 76 (95%), respectively. Additionally, the PCR analysis revealed that the frequency of virulence genes (, , , , , , and ) was 78 (97.5%), 77 (96.25%), 58 (72.5%), 77 (96.2%), 76 (95%), 31 (38.75%), and 80 (100%), respectively. Resistance to SXT isolate belong to the sequence type (ST15) and exhibits allelic profiles of (10, 29, 21, 21, 32, 32, and 10). The data obtained from our investigation have indicated that SXT remains an efficacious antibiotic and also highlighted the importance of effective management, identification of resistant isolates, and typing methods to address the global prevalence of antibiotic resistance in .
在免疫功能低下的患者中引发具有挑战性的感染，对多种抗菌药物的耐药性不断增加，并拥有多种毒力基因，包括对甲氧苄啶 - 磺胺甲恶唑的新出现的耐药性。总共从伊朗德黑兰的多家医院收集了80株临床分离株。本研究通过纸片扩散法和肉汤稀释法进行抗生素敏感性分析，通过PCR测序检测耐药性和毒力基因频率，并进行多位点测序分型（MLST）以进行菌株分型。在测试的分离株中，我们观察到亚胺培南的耐药率显著较高，为80（100%），美罗培南为78（97.5%），头孢他啶为72（90%），而甲氧苄啶 - 磺胺甲恶唑（SXT）的耐药率较低，为2（2.5%）。米诺环素和左氧氟沙星显示出最高的敏感率，分别为70（87.5%）和80（100%）。抗生素耐药基因bla 、bla 的流行率分别为71（88.75%）和76（95%）。此外，PCR分析显示毒力基因（、、、、、和）的频率分别为78（97.5%）、77（96.25%）、58（72.5%）、77（96.2%）、76（95%）、31（38.75%）和80（100%）。对SXT耐药的分离株属于序列型（ST15），并表现出（10、29、21、21、32、32和10）的等位基因谱。我们调查获得的数据表明，SXT仍然是一种有效的抗生素，同时也突出了有效管理、鉴定耐药分离株以及分型方法对于应对全球范围内抗生素耐药性流行的重要性。
