Department of Pharmacology, Therapeutics and Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos State, Nigeria.
Neuropharmacology Unit, Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria; Department of Pharmacology, Faculty of Basic Medical Sciences, PAMO University of Medical Sciences, Port Harcourt, Rivers State, Nigeria.
J Ethnopharmacol. 2019 Oct 28;243:112117. doi: 10.1016/j.jep.2019.112117. Epub 2019 Jul 24.
ETHNOPHARMACOLOGY RELEVANCE: the root decoction of Cnestis ferruginea Vahl ex DC (Connaraceae) is widely used in traditional African medicine for the treatment of various ailments including pain, inflammation and epilepsy. We have earlier reported anticonvulsant effect of Cnestis ferruginea root extract in mice.
to evaluate the effect of ethanolic root extract of Cnestis ferruginea (CF) on kainic acid (KA)-induced temporal lobe epilepsy (TLE) in mice as well as the involvement of inflammatory mediators and oxidative stress.
mice were randomly divided into preventive treatment (vehicle (normal saline) or CF (400 mg/kg, p.o.) for 3 consecutive days before KA (5 mg/kg, i.p.) on days 4 and 5. In the reversal model, KA (5 mg/kg, i.p.) was administered on days 1 and 2 before vehicle or CF (400 mg/kg) administration on days 3-5. The effect of treatments on seizure severity was recorded using Racine scale. Animals were euthanized on day 5, 6 h after last KA exposure in preventive model and 1 h after CF administration in reversal model to estimate markers of oxidative stress and neuroinflammation.
exposure of mice to KA induced TLE evidenced in increased severity of seizures which was significantly reduced by the pre- and post-treatment of mice with CF. Moreso, KA-induced malondialdehyde/nitrite generation and GSH deficit in the brain were attenuated by CF treatments. KA-induced up-regulation of inflammatory transcription factors; cyclooxygenase-2 (COX-2) and nuclear facor-kappaB (NF-κB) in the CA1, CA2, CA3 and dentate gyrus (DG) regions of the hippocampus regions were attenuated by CF treatments.
findings from this study showed that Cnestis ferruginea root extract ameliorated KA-induced TLE through enhancement of antioxidant defense mechanism and attenuation of neuro-inflammatory transcription factors. Thus, could possibly be a potential phytotherapeutic agent in the management of temporal lobe epilepsy.
民族药理学相关性：Cnestis ferruginea Vahl ex DC（卫矛科）的根煎剂在传统的非洲医学中被广泛用于治疗各种疾病，包括疼痛、炎症和癫痫。我们之前报道过 Cnestis ferruginea 根提取物在小鼠中的抗惊厥作用。
评估 Cnestis ferruginea（CF）的乙醇根提取物对小鼠海人酸（KA）诱导的颞叶癫痫（TLE）的影响，以及炎症介质和氧化应激的参与。
将小鼠随机分为预防治疗组（载体（生理盐水）或 CF（400mg/kg，口服），连续 3 天，然后在第 4 和第 5 天用 KA（5mg/kg，腹腔注射）。在逆转模型中，在第 1 和第 2 天用 KA（5mg/kg，腹腔注射）处理后，在第 3-5 天用载体或 CF（400mg/kg）处理。使用 Racine 量表记录治疗对癫痫严重程度的影响。在预防模型中，在最后一次 KA 暴露后第 5 天和 6 小时，在逆转模型中在 CF 给药后 1 小时处死动物，以估计氧化应激和神经炎症的标志物。
KA 暴露于小鼠诱导 TLE，表现为癫痫严重程度增加，用 CF 进行小鼠的预治疗和后治疗可显著降低。此外，CF 治疗可减轻 KA 诱导的脑丙二醛/亚硝酸盐生成和 GSH 缺乏。KA 诱导的海马 CA1、CA2、CA3 和齿状回（DG）区炎症转录因子环加氧酶-2（COX-2）和核因子-κB（NF-κB）的上调被 CF 治疗减弱。
本研究结果表明，Cnestis ferruginea 根提取物通过增强抗氧化防御机制和减弱神经炎症转录因子，改善了 KA 诱导的 TLE。因此，它可能是颞叶癫痫管理中的一种潜在植物治疗剂。
