群体基因组分析揭示了……中的一个多基因性别决定系统。（注：原文句末不完整，缺少具体物种等信息）

Population genomic analysis reveals a polygenic sex determination system in .

作者信息

Jiang Chunxi, Liu Shilin, Yang Yujia, Cui Wei, Xu Shuai, Rasoamananto Irène, Lavitra Thierry, Zhang Libin, Sun Lina

机构信息

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.

Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao 266237, China.

出版信息

iScience. 2024 Aug 30;27(10):110852. doi: 10.1016/j.isci.2024.110852. eCollection 2024 Oct 18.

DOI:10.1016/j.isci.2024.110852

PMID:39381746

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11458978/

Abstract

The sea cucumber , a key species in Chinese aquaculture, plays a significant evolutionary role within the Echinodermata phylum. However, the sex determination mechanism in this species remains poorly understood. Here, we conducted extensive sex surveys and sampling of eight wild populations, investigating the sex-related SNPs and insertion or deletions (indels) through bulk segregation analysis (BSA) and genome-wide association study (GWAS) analysis. Our findings suggest that employs a polygenic sex determination (PSD) system, with solute carrier family 8 (SLC8A) being the candidate gene for sex determination, encoding sodium-calcium exchanger (NCX1). The analysis of normalized sequencing depth reveals variations across chromosomes 6, 13, 14, 16, and 18, supporting the PSD system. We also identified 541.656 kb of male-specific sequences and screened five markers (C77185, C98086, C64977, C125, and C876) for molecular sex identification. Overall, this study provides new insights into sex determination, highlighting a complex multi-gene mechanism rather than a simple XX/XY system.

摘要

海参是中国水产养殖中的关键物种，在棘皮动物门中发挥着重要的进化作用。然而，该物种的性别决定机制仍知之甚少。在此，我们对八个野生种群进行了广泛的性别调查和采样，通过群体分离分析（BSA）和全基因组关联研究（GWAS）分析来研究与性别相关的单核苷酸多态性（SNP）和插入或缺失（indel）。我们的研究结果表明，海参采用多基因性别决定（PSD）系统，溶质载体家族8（SLC8A）是性别决定的候选基因，编码钠钙交换蛋白（NCX1）。对标准化测序深度的分析揭示了6号、13号、14号、16号和18号染色体上的变异，支持了PSD系统。我们还鉴定出541.656 kb的雄性特异性序列，并筛选出五个用于分子性别鉴定的标记（C77185、C98086、C64977、C125和C876）。总体而言，本研究为海参的性别决定提供了新的见解，突出了一种复杂的多基因机制，而非简单的XX/XY系统。