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通过 VVL 标记的红色染料掺杂纳米粒子检测乳腺癌和前列腺癌模型中的 Tn 抗原。

Detection of Tn-antigen in breast and prostate cancer models by VVL-labeled red dye-doped nanoparticles.

机构信息

Institute of Biomedicine, University of Turku, Turku, 20520, Finland.

Western Cancer Centre FICAN West, Turku, 20521, Finland.

出版信息

Nanomedicine (Lond). 2024;19(29):2463-2478. doi: 10.1080/17435889.2024.2405454. Epub 2024 Oct 9.

DOI:10.1080/17435889.2024.2405454
PMID:39382009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11520574/
Abstract

Fluorescence detection of breast and prostate cancer cells expressing Tn-antigen, a tumor marker, with lectin (VVL)-labeled nanoparticles. Breast and prostate cancer cells engineered to express high levels of Tn-antigen and non-engineered controls were incubated with VVL-labeled or unlabeled red dye-doped silica-coated polystyrene nanoparticles. The binding to cells was studied with flow cytometry, confocal microscopy, and electron microscopy. Flow cytometry showed that the binding of VVL-labeled nanoparticles was significantly higher to Tn-antigen-expressing cancer cells than controls. Confocal microscopy demonstrated that particles bound to the cell surface. According to the correlative light and electron microscopy the particles bound mostly as aggregates. VVL-labeled nanoparticles could provide a new tool for the detection of Tn-antigen-expressing breast and prostate cancer cells.

摘要

用凝集素（VVL）标记的纳米颗粒检测表达肿瘤标志物 Tn 抗原的乳腺癌和前列腺癌细胞的荧光。将乳腺癌和前列腺癌细胞工程化以高表达 Tn 抗原，并与非工程化对照进行孵育，用 VVL 标记或未标记的红色染料掺杂的二氧化硅涂覆聚苯乙烯纳米颗粒进行孵育。通过流式细胞术、共聚焦显微镜和电子显微镜研究与细胞的结合。流式细胞术显示，VVL 标记的纳米颗粒与表达 Tn 抗原的癌细胞的结合显著高于对照。共聚焦显微镜显示颗粒结合在细胞表面。根据相关的光镜和电镜，颗粒主要以聚集物的形式结合。VVL 标记的纳米颗粒可为检测表达 Tn 抗原的乳腺癌和前列腺癌细胞提供新工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/ee9687cea1e5/INNM_A_2405454_F0008_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/3b2bc52ea5ef/INNM_A_2405454_UF0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/f6cc31bcde47/INNM_A_2405454_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/939b8451612c/INNM_A_2405454_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/ad47f0df8894/INNM_A_2405454_F0003_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/8fce7d9ef239/INNM_A_2405454_F0004_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/5baef80146e9/INNM_A_2405454_F0005_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/87e957d29922/INNM_A_2405454_F0006_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/bfb118bd38d8/INNM_A_2405454_F0007_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/ee9687cea1e5/INNM_A_2405454_F0008_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/3b2bc52ea5ef/INNM_A_2405454_UF0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/f6cc31bcde47/INNM_A_2405454_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/939b8451612c/INNM_A_2405454_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/ad47f0df8894/INNM_A_2405454_F0003_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/8fce7d9ef239/INNM_A_2405454_F0004_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/5baef80146e9/INNM_A_2405454_F0005_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/87e957d29922/INNM_A_2405454_F0006_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/bfb118bd38d8/INNM_A_2405454_F0007_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3804/11520574/ee9687cea1e5/INNM_A_2405454_F0008_C.jpg

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