Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.
Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China.
J Agric Food Chem. 2024 Oct 23;72(42):23141-23150. doi: 10.1021/acs.jafc.4c04872. Epub 2024 Oct 9.
Cytochrome P450 monooxygenases are associated with the detoxification of xenobiotics, and overexpression of P450 genes has been proven to be associated with insecticide resistance in insect species. Our previous study has revealed that multiple CYP6AE genes were significantly overexpressed in a resistant strain of , and among these genes, was particularly highly expressed. However, the functional roles of the genes in insecticide resistance remain unknown in this pest. Here, we investigate the relationship between the CYP6AE genes and insecticide resistance by focusing on . The expression of was increased after exposure to chlorpyrifos, cypermethrin, and deltamethrin. Ectopic overexpression of P450 in transgenic flies by the GAL4/UAS system dramatically enhanced the tolerance to these three insecticides. Furthermore, the recombinant CYP6AE70 was functionally expressed in Sf9 cells, and metabolic assays revealed that the recombinant P450 protein could efficiently metabolize chlorpyrifos, cypermethrin, and deltamethrin. Finally, molecular modeling and docking also showed that this P450 protein were tightly bound to the three insecticides. These results determine that the upregulation of results in resistance to multiple insecticides in . This study improves our understanding of P450-mediated insecticide resistance and will help us to design more effective resistance management for pest control.
细胞色素 P450 单加氧酶与外来化合物的解毒有关，并且已经证明 P450 基因的过度表达与昆虫物种的杀虫剂抗性有关。我们之前的研究表明，在 的抗性品系中，多个 CYP6AE 基因显著过表达，其中 基因表达水平特别高。然而，在这种害虫中，这些 CYP6AE 基因在杀虫剂抗性中的功能作用尚不清楚。在这里，我们通过聚焦 来研究 CYP6AE 基因与杀虫剂抗性之间的关系。在接触毒死蜱、氯菊酯和溴氰菊酯后， 的表达增加。通过 GAL4/UAS 系统在转基因果蝇中外源过表达 P450 显著增强了对这三种杀虫剂的耐受性。此外，重组 CYP6AE70 在 Sf9 细胞中得到功能表达，代谢测定表明重组 P450 蛋白可以有效地代谢毒死蜱、氯菊酯和溴氰菊酯。最后，分子建模和对接也表明这种 P450 蛋白与这三种杀虫剂紧密结合。这些结果表明 的上调导致了 对多种杀虫剂的抗性。这项研究提高了我们对 P450 介导的杀虫剂抗性的理解，并将有助于我们设计更有效的害虫防治抗性管理策略。
