Biochemistry and Biotechnology Group, Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.
Microbial and Biome Sciences Group, Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.
Anal Chem. 2024 Nov 19;96(46):18343-18348. doi: 10.1021/acs.analchem.4c02835. Epub 2024 Oct 9.
Omics-based measurements enable the study of biomolecules in a high-throughput fashion, leading to the characterization and quantification of biological systems. Multi-omics methods aim to incorporate several omics measurements for a more holistic approach, which is crucial for advancing our understanding of the diversity and redundancy of biological systems. Current multi-omics sample preparation methods have achieved proteomics, lipidomics, and metabolomics from individual samples; however, the bioinformatic tools currently available for interpreting data generated from these omics are limited. Alternately, transcriptomics has a wide arsenal of available bioinformatic tools offering intensive sample characterization but has yet to be incorporated into a unified, multi-omics sample preparation technique. Herein we describe the modified bead-enabled accelerated monophasic multi-omics (mBAMM) method, which incorporates RNA extraction for transcriptomics analysis. mBAMM was shown to enable RNA-seq without compromising the isolation of biomolecules for proteomics, lipidomics, and metabolomics. This methodology greatly improves sample characterization and represents a major innovation toward cohesive insights into biological systems.
基于组学的测量方法能够以高通量的方式研究生物分子，从而对生物系统进行特征描述和定量分析。多组学方法旨在整合多种组学测量方法，以实现更全面的研究方法，这对于深入了解生物系统的多样性和冗余性至关重要。目前的多组学样品制备方法已经可以从单个样品中获得蛋白质组学、脂质组学和代谢组学数据；然而，目前可用于解释这些组学数据的生物信息学工具有限。相反，转录组学拥有广泛的可用生物信息学工具，可以对样品进行深入的特征描述，但尚未整合到统一的多组学样品制备技术中。在这里，我们描述了改良的珠子加速单相多组学（mBAMM）方法，该方法可用于转录组学分析的 RNA 提取。mBAMM 能够在不影响蛋白质组学、脂质组学和代谢组学生物分子分离的情况下进行 RNA-seq。这种方法极大地提高了样品的特征描述能力，代表了对生物系统有凝聚力的见解的重大创新。
