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探讨侧基带有弹性蛋白样肽重复单元的支化分子的 LCST 和 UCST 类似行为。

Exploring LCST- and UCST-like Behavior of Branched Molecules Bearing Repeat Units of Elastin-like Peptides as Side Components.

机构信息

Department of Chemistry, Faculty and Graduate School of Science, Kyushu University, Fukuoka 819-0395, Japan.

Faculty of Arts and Science, Kyushu University, Fukuoka 819-0395, Japan.

出版信息

Biomacromolecules. 2024 Nov 11;25(11):7156-7166. doi: 10.1021/acs.biomac.4c00751. Epub 2024 Oct 9.

DOI:10.1021/acs.biomac.4c00751
PMID:39383337
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11558673/
Abstract

Elastin-like peptides (ELPs) exhibit lower critical solution temperature (LCST)-type behavior, being soluble at low temperatures and insoluble at high temperatures. While the properties of linear, long-chain ELPs are well-studied, short-chain ELPs, especially those with branched architectures, have been less explored. Herein, to obtain further insights into multimeric short ELPs, we investigated the temperature-responsive properties of branched molecules composed of a repeating pentapeptide unit of short ELPs, Phe-Pro-Gly-Val-Gly, as side components and oligo(Glu) as a backbone structure. In turbidimetry experiments, the branched ELPs showed LCST-like behavior similar to conventional ELPs and upper critical solution temperature (UCST)-like behavior, which are rarely observed in ELPs. In addition, the morphological aspects and mechanisms underlying the temperature-responsiveness were investigated. We observed that spherical aggregates formed, and the branched ELPs underwent structural changes through the self-assembly process. This study demonstrates the unique temperature-responsiveness of branched short ELPs, providing new insights into the future development and use of ELPs with tailored properties.

摘要

弹性蛋白样肽 (ELPs) 表现出较低临界溶解温度 (LCST) 型行为，即在低温下可溶，在高温下不可溶。虽然线性长链 ELPs 的性质已经得到了很好的研究，但短链 ELPs，特别是具有支化结构的短链 ELPs，研究得较少。在此，为了更深入地了解多聚短 ELPs，我们研究了由短 ELPs 的重复五肽单元（Phe-Pro-Gly-Val-Gly）作为侧基和寡聚（Glu）作为主链结构组成的支化分子的温度响应特性。在浊度实验中，支化 ELPs 表现出类似于传统 ELPs 的 LCST 型行为和很少在 ELPs 中观察到的上临界溶解温度 (UCST) 型行为。此外，还研究了温度响应的形态方面和机制。我们观察到形成了球形聚集体，并且支化 ELPs 通过自组装过程发生了结构变化。这项研究展示了支化短 ELPs 的独特温度响应性，为具有定制特性的 ELPs 的未来开发和应用提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c189/11558673/d0faa209fcd9/bm4c00751_0008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c189/11558673/3bfa62a3aa35/bm4c00751_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c189/11558673/1c2bb8ba48b3/bm4c00751_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c189/11558673/b42ed0fd25a5/bm4c00751_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c189/11558673/f47f3bb13433/bm4c00751_0004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c189/11558673/f4ee4e2b379c/bm4c00751_0006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c189/11558673/d0faa209fcd9/bm4c00751_0008.jpg

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本文引用的文献

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Biomacromolecules. 2023 Nov 13;24(11):5265-5276. doi: 10.1021/acs.biomac.3c00779. Epub 2023 Oct 22.
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Synergistic Approaches in the Design and Applications of UCST Polymers.相转变聚合物的设计与应用中的协同方法。
Macromol Rapid Commun. 2023 Dec;44(23):e2300261. doi: 10.1002/marc.202300261. Epub 2023 Jul 28.
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Development of truncated elastin-like peptide analogues with improved temperature-response and self-assembling properties.
开发具有改进的温度响应和自组装性能的截短弹性蛋白样肽类似物。
Sci Rep. 2022 Nov 12;12(1):19414. doi: 10.1038/s41598-022-23940-0.
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Branched short elastin-like peptides with temperature responsiveness obtained by EDTA-mediated multimerization.通过EDTA介导的多聚化获得的具有温度响应性的支链短弹性蛋白样肽。
J Pept Sci. 2023 Feb;29(2):e3449. doi: 10.1002/psc.3449. Epub 2022 Sep 9.
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Self-assembling systems comprising intrinsically disordered protein polymers like elastin-like recombinamers.由诸如弹性蛋白样重组蛋白等内在无序蛋白质聚合物组成的自组装系统。
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Structure-Property Relationships of Elastin-like Polypeptides: A Review of Experimental and Computational Studies.类弹性蛋白多肽的结构-性质关系：实验与计算研究综述
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