Mondal Naba Kumar, Mondal Barnali, Koley Rajesh, Koley Apurba, Balachandran Srinivasan
Environmental Chemistry Laboratory, Department of Environmental Science, The University of Burdwan, Burdwan, West Bengal, India.
Environmental Chemistry Laboratory, Department of Environmental Science, The University of Burdwan, Burdwan, West Bengal, India.
J Trace Elem Med Biol. 2024 Dec;86:127541. doi: 10.1016/j.jtemb.2024.127541. Epub 2024 Oct 3.
Arsenic migration from soil to crop plants and subsequently human consumption of contaminated foodstuffs is a serious threat for society. In the present study, two oxidation states of selenium [Se(0) and Se(VI)] were used to check their efficacy towards amelioration of arsenic toxicity in chickpeas (Cicer arietinum L.). Three different concentrations (1, 5, and 10 mg/L) of both oxidation states of selenium were applied separately and in combination against a fixed dose (10 mg/L) of arsenic [(As(V)] treatment on chickpea seedlings. Further, seed germination and seedling growth attributes, oxidative stress, and antioxidant defense under different treatments were analyzed. The changes in anatomical structures and arsenic accumulation in different parts of seedlings were also studied. Results revealed that increased generation of oxidative stress affected physiobiochemical parameters of seedlings and diminished plant growth and deformation in vascular bundles under arsenic stress. However, the combined application of Se with As showed overall improvement in seedling growth, reduced oxidative stress, and organized vascular bundles of chickpea seedlings as compared to arsenic stress alone. The arsenic uptake and accumulation in chickpea seedlings were also reduced upon supplementation of Se. The highest reduction of arsenic accumulation by 42 and 56 % in roots, while 47 and 58 % in shoots were recorded by the application of 10 mg/L of Se(0) and Se (VI) under As stress, respectively. Overall, Se(VI) showed much better performance towards the minimization of arsenic-induced phytotoxicity and arsenic accumulation as compared to Se(0). Therefore, this study explored the efficacy of different forms of selenium towards the mitigation of arsenic toxicity in plants.
砷从土壤迁移至农作物，进而被人类食用受污染的食物，这对社会构成了严重威胁。在本研究中，使用了两种氧化态的硒[Se(0)和Se(VI)]来检验它们对缓解鹰嘴豆（Cicer arietinum L.）砷毒性的效果。分别以三种不同浓度（1、5和10mg/L）的两种氧化态硒单独及组合施用，以对抗固定剂量（10mg/L）的砷[(As(V)]对鹰嘴豆幼苗的处理。此外，还分析了不同处理下的种子萌发和幼苗生长特性、氧化应激及抗氧化防御。同时研究了幼苗不同部位的解剖结构变化和砷积累情况。结果表明，氧化应激的增加影响了幼苗的生理生化参数，降低了植物生长，并使砷胁迫下维管束变形。然而，与单独的砷胁迫相比，硒与砷的组合施用总体上改善了鹰嘴豆幼苗的生长，降低了氧化应激，并使维管束结构有序。补充硒后，鹰嘴豆幼苗对砷的吸收和积累也有所减少。在砷胁迫下，分别施用10mg/L的Se(0)和Se(VI)时，根部砷积累的最大降幅分别为42%和56%，地上部为47%和58%。总体而言，与Se(0)相比，Se(VI)在最小化砷诱导的植物毒性和砷积累方面表现更好。因此，本研究探讨了不同形式的硒对减轻植物砷毒性的效果。
