肺栓塞中氧化脂质和脂质介质的鉴定。
Identification of oxylipins and lipid mediators in pulmonary embolism.
机构信息
Department of Clinical Laboratory, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, China.
Department of Clinical Laboratory Medicine, Affiliated Hospital of Medical School, Taikang Xianlin Drum Tower Hospital, Nanjing University, Nanjing, 210008, China.
出版信息
Lipids Health Dis. 2024 Oct 9;23(1):330. doi: 10.1186/s12944-024-02315-6.
BACKGROUND
This study aimed to investigate the role of oxylipins and lipid mediators in Pulmonary Embolism (PE), a serious cardiovascular condition associated with high morbidity and mortality rates.
METHODS
A total of 6,365 hospitalized patients with thrombosis and 200 healthy individuals were recruited as the control group from 2015 to 2023. Thrombus type, coagulation, and lipid-related parameters were statistically analysed. Additionally, lipidomic characteristics of serum samples from the PE and control groups were examined via LC-MS/MS for the first time.
RESULTS
Among the 6,365 hospitalized patients with thrombosis, 72.1% (4,587/6,365) had venous thromboembolism (VTE). Within the VTE group, the incidence of PE was 12.1% (555/4,587). In comparison to the healthy control (HC) group, the PE group exhibited significant elevations in coagulation-related parameters, such as factor VIII (F VIII) and von Willebrand factor (vWF) activities, while antithrombin III (AT III) and factor XII (F XII) activities were notably reduced. Lipid-related parameters, including serum cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A (apoA), were significant reductions in PE patients (P < 0.0001), with areas under the curve (AUCs) exceeding 0.9. LC-MS/MS analysis of serum samples revealed 118 oxidized lipid metabolites. Compared to the HC group, the PE group exhibited 10 upregulated oxidized lipid metabolites, with the most significant difference observed in 20-hydroxyPGF2α derived from arachidonic acid (ARA). The study identified upregulated oxidized lipid metabolites primarily linked to the ARA metabolism signalling pathway.
CONCLUSION
This research indicates a notable correlation between lipid metabolism and the occurrence and development of PE. Specifically, upregulation of the arachidonic acid metabolism signalling pathway may be an important pathogenic factor for PE, and 20-hydroxyPGF2α derived from ARA has potential as a biomarker for PE disease.
背景
本研究旨在探讨氧化脂质和脂质介质在肺栓塞（PE）中的作用，PE 是一种严重的心血管疾病，发病率和死亡率都很高。
方法
从 2015 年至 2023 年，共招募了 6365 名患有血栓的住院患者和 200 名健康个体作为对照组。对血栓类型、凝血和脂质相关参数进行了统计学分析。此外，还首次通过 LC-MS/MS 检测了 PE 组和对照组血清样本的脂质组学特征。
结果
在 6365 名患有血栓的住院患者中，72.1%（4587/6365）患有静脉血栓栓塞症（VTE）。在 VTE 组中，PE 的发病率为 12.1%（555/4587）。与健康对照组（HC）相比，PE 组的凝血相关参数明显升高，如因子 VIII（F VIII）和血管性血友病因子（vWF）活性，而抗凝血酶 III（AT III）和因子 XII（F XII）活性显著降低。脂质相关参数，包括血清胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、载脂蛋白 A（apoA），在 PE 患者中显著降低（P<0.0001），曲线下面积（AUC）超过 0.9。对血清样本的 LC-MS/MS 分析显示 118 种氧化脂质代谢物。与 HC 组相比，PE 组有 10 种上调的氧化脂质代谢物，其中最显著的差异是来源于花生四烯酸（ARA）的 20-羟前列腺素 F2α（20-hydroxyPGF2α）。研究发现，上调的氧化脂质代谢物主要与 ARA 代谢信号通路有关。
结论
本研究表明脂质代谢与 PE 的发生和发展之间存在显著相关性。具体来说，花生四烯酸代谢信号通路的上调可能是 PE 的一个重要致病因素，而来源于 ARA 的 20-羟前列腺素 F2α 可能是 PE 疾病的潜在生物标志物。
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